Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | reuired. About 1% residual activity in absence of 2-mercaptoethanol | Aequorea victoria |
Cloned (Comment) | Organism |
---|---|
- |
Aequorea victoria |
Protein Variants | Comment | Organism |
---|---|---|
C145S | 61% of wild-type activity | Aequorea victoria |
C145S/C152S | 1% of wild-type activity | Aequorea victoria |
C145S/C152S/C180S | 21% of wild-type activity. Contrary to wild-type, 49% residual activity in absence of 2-mercaptoethanol. The regeneration of the triple mutant aequorin is sharply inhibited by 2-mercaptoethanol | Aequorea victoria |
C145S/C180S | 68% of wild-type activity. Contrary to wild-type, 28% residual activity in absence of 2-mercaptoethanol | Aequorea victoria |
C152S | 46% of wild-type activity | Aequorea victoria |
C152S/C180S | 17% of wild-type activity. Contrary to wild-type, 14% residual activity in absence of 2-mercaptoethanol | Aequorea victoria |
C180S | 13% of wild-type activity | Aequorea victoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aequorea victoria | P02592 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | - |
Aequorea victoria | coelenteramide + CO2 + hnu | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Aequorea victoria |