Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.12.24 extracted from

  • Kurose, K.; Inouye, S.; Sakaki, Y.; Tsuji, F.I.
    Bioluminescence of the Ca2+-binding photoprotein aequorin after cysteine modification (1989), Proc. Natl. Acad. Sci. USA, 86, 80-84 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol reuired. About 1% residual activity in absence of 2-mercaptoethanol Aequorea victoria

Cloned(Commentary)

Cloned (Comment) Organism
-
Aequorea victoria

Protein Variants

Protein Variants Comment Organism
C145S 61% of wild-type activity Aequorea victoria
C145S/C152S 1% of wild-type activity Aequorea victoria
C145S/C152S/C180S 21% of wild-type activity. Contrary to wild-type, 49% residual activity in absence of 2-mercaptoethanol. The regeneration of the triple mutant aequorin is sharply inhibited by 2-mercaptoethanol Aequorea victoria
C145S/C180S 68% of wild-type activity. Contrary to wild-type, 28% residual activity in absence of 2-mercaptoethanol Aequorea victoria
C152S 46% of wild-type activity Aequorea victoria
C152S/C180S 17% of wild-type activity. Contrary to wild-type, 14% residual activity in absence of 2-mercaptoethanol Aequorea victoria
C180S 13% of wild-type activity Aequorea victoria

Organism

Organism UniProt Comment Textmining
Aequorea victoria P02592
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2
-
Aequorea victoria coelenteramide + CO2 + hnu
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Aequorea victoria