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Literature summary for 1.13.12.24 extracted from
- Red fluorescent protein-aequorin fusions as improved bioluminescent Ca2+ reporters in single cells and mice (2011), PLoS ONE, 6, e19520 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | fusion of aequorin to orange and various red fluorescent proteins to identify the best acceptor in red emission bands. Tandem-dimer tomato-aequorin shows the highest BRET efficiency (largest energy transfer critical distance R0) and percentage of counts in the red band of all the fusions studied. Light output is sufficient to image ATP-induced Ca2+ oscillations in single HeLa cells expressing tandem-dimer tomato-aequorin. Ca2+ rises caused by depolarization of mouse neuronal cells in primary culture are also recorded, and changes in fine neuronal projections are spatially resolved. It is possible to visualize the Ca2+ activity of HeLa cells injected subcutaneously into mice, and Ca2+ signals after depositing recombinant tandem-dimer tomato-aequorin in muscle or the peritoneal cavity | Aequorea victoria |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Aequorea victoria |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aequorea victoria | - |
- |
- |
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Release 2023.1 (January 2023)
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