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Literature summary for 1.13.12.24 extracted from

  • Shimomura, O.; Johnson, F.H.; Saiga, Y.
    Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea (1962), J. Cell. Comp. Physiol., 59, 223-239 .
    View publication on PubMed
Search for more literature for 1.13.12.24

General Stability

General Stability Organism
protein is relatively stable in 0.01 M Na2EDTA Aequorea forskalea

Inhibitors

Inhibitors Comment Organism Structure
Barbiturate 50 mM, complete loss of activity Aequorea forskalea
Cyanate 50 mM, complete loss of activity Aequorea forskalea
EDTA
-
 Aequorea forskalea
glutathione 10 mM, complete loss of activity Aequorea forskalea
H2O2 50 mM, complete loss of activity Aequorea forskalea
malonate 50 mM, complete loss of activity Aequorea forskalea
NaAsO2 20 mM, complete loss of activity Aequorea forskalea
nitrite 50 mM, complete loss of activity Aequorea forskalea
thiocyanate 50 mM, complete loss of activity Aequorea forskalea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for luminescence reaction Aequorea forskalea

Organic Solvent Stability

Organic Solvent Comment Organism
benzyl alcohol 50 mM, 10% increase in activity Aequorea forskalea
n-amyl alcohol 50 mM, 7% increase in activity Aequorea forskalea
n-Butanol 50 mM, 8% increase in activity Aequorea forskalea
n-heptanol 50 mM, 30% increase in activity Aequorea forskalea
n-hexanol 50 mM, 10% increase in activity Aequorea forskalea
n-octanol 50 mM, 18% increase in activity Aequorea forskalea

Organism

Organism UniProt Comment Textmining
Aequorea forskalea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aequorea forskalea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1 8.3
-
 Aequorea forskalea

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 unstable below Aequorea forskalea