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Literature summary for 1.13.12.22 extracted from

  • Machovina, M.M.; Usselman, R.J.; DuBois, J.L.
    Monooxygenase substrates mimic flavin to catalyze cofactorless oxygenations (2016), J. Biol. Chem., 291, 17816-17828 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
N18A the mutation suppresses the kcat value 120fold compared to the wild type enzyme Streptomyces nogalater
N63A the mutation suppresses the kcat value 50fold compared to the wild type enzyme Streptomyces nogalater

Inhibitors

Inhibitors Comment Organism Structure
bisanthrone
-
Streptomyces nogalater
dithranone
-
Streptomyces nogalater

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
dithranol at pH 9.8 and 20°C Streptomyces nogalater
0.22
-
dithranol at pH 6.3 and 20°C Streptomyces nogalater
0.41
-
O2 at pH 9.8 and 20°C Streptomyces nogalater
0.43
-
O2 at pH 6.3 and 20°C Streptomyces nogalater

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynogalonate + O2 Streptomyces nogalater
-
nogalonate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces nogalater O54259
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynogalonate + O2
-
Streptomyces nogalater nogalonate + H2O
-
?
dithranol + O2
-
Streptomyces nogalater dithranolate + H2O
-
?

Subunits

Subunits Comment Organism
? x * 16980, calculated from amino acid sequence Streptomyces nogalater

Synonyms

Synonyms Comment Organism
SnoaB
-
Streptomyces nogalater

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
O2 at pH 6.3 and 20°C Streptomyces nogalater
0.17
-
dithranol at pH 6.3 and 20°C Streptomyces nogalater
1
-
dithranol at pH 9.8 and 20°C Streptomyces nogalater
1.83
-
O2 at pH 9.8 and 20°C Streptomyces nogalater

General Information

General Information Comment Organism
physiological function involved in nogalamycin biosynthesis Streptomyces nogalater

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.5
-
dithranol at pH 6.3 and 20°C Streptomyces nogalater
0.83
-
dithranol at pH 6.3 and 20°C Streptomyces nogalater
4.3
-
dithranol at pH 9.8 and 20°C Streptomyces nogalater
9.7
-
dithranol at pH 9.8 and 20°C Streptomyces nogalater