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Literature summary for 1.13.12.19 extracted from
- Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. Phaseolicola PK2 (1991), J. Gen. Microbiol., 137, 2281-2286.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-arginine | 3% of the activity with L-arginine | Pseudomonas syringae |  |
| L-arginine | highly specific for cofactor L-arginine, KM value 0.018 mM | Pseudomonas syringae | |
| L-canavanine sulfate | 7% of the activity with L-arginine | Pseudomonas syringae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4,5-dihydroxy-1,3-benzene disulfonic acid | 1 mM, 0.8% residual activity | Pseudomonas syringae | |
| 5,5'-dithio-bis(2-nitrobenzoate) | 1 mM, 0.7% residual activity | Pseudomonas syringae | |
| CoCl2 | 1 mM, 20% residual activity | Pseudomonas syringae | |
| CuSO4 | 1 mM, 50% residual activity | Pseudomonas syringae | |
| EDTA | 1 mM, 1% residual activity | Pseudomonas syringae | |
| H2O2 | 1 mM, 0.7% residual activity | Pseudomonas syringae | |
| MnCl2 | 1 mM, 6% residual activity | Pseudomonas syringae | |
| n-propyl gallate | 1 mM, 1% residual activity | Pseudomonas syringae | |
| Sodium azide | 1 mM, 90% residual activity | Pseudomonas syringae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
2-oxoglutarate | pH 8.0, 25°C | Pseudomonas syringae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required, KM value 0.059 mM | Pseudomonas syringae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel fitlration | Pseudomonas syringae |
| 42000 | - |
1 * 42000, SDS-PAGE | Pseudomonas syringae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas syringae | P32021 | pv. phaseolicola PK2, enzyme catalyzes the formation of ethylene and succinate from 2-oxoglutarate, reactions of EC 1.13.12.19 and EC 1.14.11.34 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from cell-free extract | Pseudomonas syringae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 660 | - |
pH 8.0, 25°C | Pseudomonas syringae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + O2 | enzyme is highly specific for substrate 2-oxoglutarate | Pseudomonas syringae | ethylene + 3 CO2 + H2O | - |
? | |
| additional information | presence of 2-oxoglutarate, L-arginine, Fe2+ and oxygen is essential for the enzymic reaction | Pseudomonas syringae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Pseudomonas syringae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 25 | - |
Pseudomonas syringae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below | Pseudomonas syringae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Pseudomonas syringae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas syringae | isoelectric focusing | - |
5.9 |
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