Cloned (Comment) | Organism |
---|---|
deletion mutants for N- or C-terminal regions of domain 3 of the luciferase are expressed in Escherichia coli BL21(DE3) cells | Lingulodinium polyedra |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutants for N-terminal region of domain 3 of the luciferase show above 20% of wild type activity, the activities of C-terminal deleted mutants decrease drastically to below 1% of wild type | Lingulodinium polyedra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Lingulodinium polyedra |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dinoflagellate luciferin + O2 | Lingulodinium polyedra | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lingulodinium polyedra | - |
formerly known as Gonyaulax polyedra | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Lingulodinium polyedra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra | ? | - |
? | |
dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra | oxidized dinoflagellate luciferin + H2O + hv | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 2 * 42000, SDS-PAGE | Lingulodinium polyedra |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
the optimum pH of Lingulodinium polyedrum luciferase and for each of its domains is approximately 6.0 | Lingulodinium polyedra |
6.5 | - |
Lingulodinium polyedrum luciferase domain 3 | Lingulodinium polyedra |