Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Streptomyces ansochromogenes |
Crystallization (Comment) | Organism |
---|---|
crystal structure of NAO from Streptomyces ansochromogenes is determined. It consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern | Streptomyces ansochromogenes |
Protein Variants | Comment | Organism |
---|---|---|
H179D | mutant enzyme shows no activity. Crystal structure of mutant H179D is determined: The structural superimposition of wild-type Sa-NAO and mutant H179D-nitroethane shows no significant structural variation | Streptomyces ansochromogenes |
H179K | mutant enzyme shows no activity | Streptomyces ansochromogenes |
H179V | mutant enzyme shows no activity | Streptomyces ansochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces ansochromogenes | Q9FDD4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-nitropropane + O2 | - |
Streptomyces ansochromogenes | propionaldehyde + HNO2 | - |
? | |
2-nitropropane + O2 | - |
Streptomyces ansochromogenes | acetone + HNO2 | - |
? | |
nitroethane + O2 | - |
Streptomyces ansochromogenes | acetaldehyde + HNO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nitroalkane oxidase | - |
Streptomyces ansochromogenes |
Sa-NAO | - |
Streptomyces ansochromogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces ansochromogenes |