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Literature summary for 1.13.12.16 extracted from

  • Kido, T.; Soda, K.; Asada, K.
    Properties of 2-nitropropane dioxygenase of Hansenula mrakii. Formation and participation of superoxide (1978), J. Biol. Chem., 253, 226-232.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
3-(3,4-dihydroxyphenyl)-L-Ala
-
 Cyberlindnera mrakii
5-hydroxytryptophan
-
 Cyberlindnera mrakii
acetone inhibits competitively against 2-nitropropane, no inhibitory effect against O2 Cyberlindnera mrakii
alpha-Naphthol
-
 Cyberlindnera mrakii
catechol
-
 Cyberlindnera mrakii
cytochrome c competitive inhibitor Cyberlindnera mrakii
epinephrine competitive inhibitor Cyberlindnera mrakii
hydroquinone
-
 Cyberlindnera mrakii
additional information inhibition by superoxide dismutase and various scavengers for superoxide; phenol, tryptophan, and tryptamine show no effect on the reaction Cyberlindnera mrakii
NADH competitive inhibitor. Oxygenation is inhibited approximately 86% by 0.1 mM NADH, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized Cyberlindnera mrakii
NADPH competitive inhibitor. oxygenation is inhibited approximately 100% by 0.1 mM NADPH, respectively, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized Cyberlindnera mrakii
nitrite inhibits noncompetitively against 2-nitropropane, functioned as a noncompetitive inhibitor against oxygen in the presence of excess 2-nitropropane (50 mM) Cyberlindnera mrakii
phloroglucinol
-
 Cyberlindnera mrakii
pyrogallol
-
 Cyberlindnera mrakii
resorcinol
-
 Cyberlindnera mrakii
serotonin
-
 Cyberlindnera mrakii
Superoxide dismutase
-
 Cyberlindnera mrakii
Thiophenol
-
 Cyberlindnera mrakii
Tiron i.e. pyrocatechol-3,5-disulfonate disodium salt Cyberlindnera mrakii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.277
-
 O2
-
 Cyberlindnera mrakii
19.2
-
 2-Nitropropane
-
 Cyberlindnera mrakii

Organism

Organism UniProt Comment Textmining
Cyberlindnera mrakii
-
-
-
Cyberlindnera mrakii
-
 IF0 0895
-
Cyberlindnera mrakii IF0 0895
-
 IF0 0895
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity Cyberlindnera mrakii

Reaction

Reaction Comment Organism Reaction ID
ethylnitronate + O2 = acetaldehyde + nitrite + other products superoxide as reactive intermediate Cyberlindnera mrakii
a
ethylnitronate + O2 = acetaldehyde + nitrite + other products ordered bi bi mechanism in which 2-nitropropane first combines with the enzyme and the enzyme 2-nitropropane complex reacts with oxygen to form a ternary complex Cyberlindnera mrakii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitropropane + O2 superoxide as reactive intermediate Cyberlindnera mrakii acetone + HNO2
-
 ?
2-nitropropane + O2 superoxide as reactive intermediate Cyberlindnera mrakii IF0 0895 acetone + HNO2
-
 ?

Synonyms

Synonyms Comment Organism
2-nitropropane dioxygenase
-
 Cyberlindnera mrakii

Cofactor

Cofactor Comment Organism Structure
cytochrome c cytochrome c also inhibits the reaction and half-inhibition is found in the presence of 17 microM cytochrome c. The addition of cytochrome c to the reaction mixture containing 2-nitropropane and 2-nitropropane dioxygenase causes an increase in absorbance at 550 nm indicating the reduction of cytochrome c Cyberlindnera mrakii