Cloned (Comment) | Organism |
---|---|
recombinant expression and secretion of 19 kDa catalytic subunit mutant nanoKAZ from CHO-K1 cells. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway. Recombinant expression of His-tagged wild-type and mutant KAZs in Escherichia coli. The secreted proteins form aggregates | Oplophorus gracilirostris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway, Construction of other DELTA1-15 truncated mutants of the catalytic subunit KAZ, i.e. DN2T, DN3L, DN4E, DN5D, DN6F, DN7V, DN8G, DN9D, DN10W, DN15G, and DN20Q. The mutant enzyme activity is dependent on the mutation and the type of expression vector, overview. Secretory expression of nanoKAZ from mammalian cells in the absence of the N-terminal signal peptide | Oplophorus gracilirostris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme possesses an amino-terminal signal peptide and is secreted | Oplophorus gracilirostris | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
full-length enzyme | Oplophorus gracilirostris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | Oplophorus gracilirostris | - |
coelenteramide + CO2 + hv | - |
? | |
Oplophorus luciferin + O2 | Oplophorus gracilirostris | - |
oxidized Oplophorus luciferin + CO2 + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oplophorus gracilirostris | Q9GV45 AND Q9GV46 | catalytic and noncatalytic subunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | - |
Oplophorus gracilirostris | coelenteramide + CO2 + hv | - |
? | |
Oplophorus luciferin + O2 | - |
Oplophorus gracilirostris | oxidized Oplophorus luciferin + CO2 + hv | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterooligomer | x * 19000, catalytic subunit, + x * 35000, noncatalytic subunit, SDS-PAGE | Oplophorus gracilirostris |
Synonyms | Comment | Organism |
---|---|---|
KAZ | catalytic enzyme subunit | Oplophorus gracilirostris |
Oplophorus luciferase | - |
Oplophorus gracilirostris |
General Information | Comment | Organism |
---|---|---|
malfunction | recombinant mutant nanoKAZ lacks the amino-terminal signal peptide is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway | Oplophorus gracilirostris |