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Literature summary for 1.13.12.13 extracted from

  • Inouye, S.; Sahara, Y.
    Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system (2008), Biochem. Biophys. Res. Commun., 376, 448-453.
    View publication on PubMed

Application

Application Comment Organism
molecular biology useful reporter protein in various assay systems including reporter assays and immunoassays Oplophorus gracilirostris

Cloned(Commentary)

Cloned (Comment) Organism
construction of a cold induced expression vector in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of protein A (ZZ-domain) and the multiple cloning sites, the role of ZZ-domain as a solubilizing partner at 15°C is demonstrated by expressing the imidazopyrazinone-type luciferase of Oplophorus Oplophorus gracilirostris

Organism

Organism UniProt Comment Textmining
Oplophorus gracilirostris Q9GV45
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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0.1% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), precipitate Oplophorus gracilirostris
additional information
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100% luminescence activity expressed in Escherichia coli cells using pCold-ZZ-KAZ vector (with IgG-binding domain of protein A), supernatant Oplophorus gracilirostris
additional information
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2.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), supernatant Oplophorus gracilirostris
additional information
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4.7% luminescence activity expressed in Escherichia coli cells using pCold-KAZ vector (without IgG-binding domain of protein A), precipitate Oplophorus gracilirostris

Synonyms

Synonyms Comment Organism
imidazopyrazinone-type luciferase
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Oplophorus gracilirostris
Oplophorus luciferase
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Oplophorus gracilirostris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
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assay at Oplophorus gracilirostris