Literature summary for 1.13.11.90 extracted from

Rajakovich, L.; Pandelia, M.; Mitchell, A.; Chang, W.; Zhang, B.; Boal, A.; Krebs, C.; Bollinger, J.
A new microbial pathway for organophosphonate degradation catalyzed by two previously misannotated non-heme-iron oxygenases (2019), Biochemistry, 58, 1627-1647 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Leisingera caerulea

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with Fe2+ and [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate, hanging drop vapor diffusion method, using 0.2 M CaCl2, 0.1 M sodium HEPES (pH 7.5), and 27-33% polyethylene glycol 4000	Leisingera caerulea

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	the enzyme uses a mixed-valent diiron cofactor	Leisingera caerulea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2	Leisingera caerulea	-	glycine betaine + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Leisingera caerulea	A0A4V8H040	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Sephadex S-200 gel filtration	Leisingera caerulea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2	-	Leisingera caerulea	glycine betaine + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
tmpB	-	Leisingera caerulea

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Release 2023.1 (January 2023)