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Literature summary for 1.13.11.88 extracted from

  • Tang, J.; Shi, L.; Li, L.; Long, L.; Ding, S.
    Expression and characterization of a 9-cis-epoxycarotenoid dioxygenase from Serratia sp. ATCC 39006 capable of biotransforming isoeugenol and 4-vinylguaiacol to vanillin (2018), Biotechnol. Rep., 18, e00253 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.88

Activating Compound

Activating Compound Comment Organism Structure
Trichloromethane the enzyme activity is substantially improved at presence of 10% (v/v) of trichloromethane (176.16% activity with isoeugenol and 208.47% activity with 4-vinylguaiacol) Serratia sp. ATCC 39006

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Serratia sp. ATCC 39006

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 66.54% residual activity in the presence of 5 mM 1,10-phenanthroline Serratia sp. ATCC 39006
Cu2+ severe inhibition (3.17% residual activity) at 5 mM Cu2+ Serratia sp. ATCC 39006
EDTA 64.77% residual activity in the presence of 5 mM EDTA Serratia sp. ATCC 39006
ethyl acetate in the presence 10% (v/v) ethyl acetate, the enzyme shows 19.35% residual activity with isoeugenol and 35.06% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
Fe2+ 70.55% residual activity in the presence of 5 mM Fe2+ Serratia sp. ATCC 39006
Fe3+ 62.51% residual activity in the presence of 5 mM Fe3+ Serratia sp. ATCC 39006
additional information not significantly inhibited by SDS and 2,2-bipyridine Serratia sp. ATCC 39006
n-Butyl alcohol in the presence of 10% (v/v) n-butyl alcohol, the enzyme shows 38.19% residual activity with isoeugenol and 21.44% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
n-caprylic alcohol in the presence of 10% (v/v) n-caprylic acid, the enzyme shows 74.73% residual activity with isoeugenol as substrate Serratia sp. ATCC 39006
n-octane in the presence of 10% (v/v) n-octane, the enzyme shows 55.34% residual activity with isoeugenol and 70.45% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
Ni2+ 74.61% residual activity in the presence of 5 mM Ni2+ Serratia sp. ATCC 39006
petroleum ether in the presence of 10% (v/v) petroleum ether, the enzyme shows 46.05% residual activity with isoeugenol as substrate Serratia sp. ATCC 39006
Toluene in the presence of 10% (v/v) toluene, the enzyme shows 39.56% residual activity with isoeugenol and 29.36% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
Triton X-100 66.54% residual activity in the presence of 5 mM Triton X-100 Serratia sp. ATCC 39006
Zn2+ 77.07% residual activity in the presence of 5 mM Zn2+ Serratia sp. ATCC 39006

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.31
-
 4-vinylguaiacol at pH 8.0 and 30°C Serratia sp. ATCC 39006
18.92
-
 isoeugenol at pH 8.0 and 30°C Serratia sp. ATCC 39006

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulates the enzyme activity Serratia sp. ATCC 39006
additional information no significant changes of enzyme activity with Ca2+, Co2+, Mg2+, Li+, Mn2+ and Na+ Serratia sp. ATCC 39006

Organism

Organism UniProt Comment Textmining
Serratia sp. ATCC 39006 A0A2I5TAF0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Serratia sp. ATCC 39006

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-vinylguaiacol + O2 32% conversion Serratia sp. ATCC 39006 vanillin + formaldehyde
-
 ?
isoeugenol + O2 92% conversion Serratia sp. ATCC 39006 vanillin + acetaldehyde
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Serratia sp. ATCC 39006
? x * 55590, calculated from amino acid sequence Serratia sp. ATCC 39006

Synonyms

Synonyms Comment Organism
NCED
-
 Serratia sp. ATCC 39006

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Serratia sp. ATCC 39006

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 the enzyme presents over 80% of relative activity under a temperature range from 20 to 40°C, but almost totally inactivation as the temperature is above 45°C Serratia sp. ATCC 39006

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 at 20°C and 30°C, more than 90% of initial activity residue is detected after 150 min incubation. However, its activity is decreased when incubated at temperatures higher than 40°C, about 50% of its initial activity is detected at 40°C after 30 min of incubation and almost inactivated at 50°C Serratia sp. ATCC 39006

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Serratia sp. ATCC 39006

pH Range

pH Minimum pH Maximum Comment Organism
7 9 over 80% of relative activity is detected under a pH range from 7.0 to 9.0 Serratia sp. ATCC 39006

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 the enzyme shows a high stability at pH between 5.0 and 11.0, over 80% of initial activity is maintained after 2 h of incubation at universal buffers Serratia sp. ATCC 39006

pI Value

Organism Comment pI Value Maximum pI Value
Serratia sp. ATCC 39006 calculated from amino acid sequence
-
 5