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Literature summary for 1.13.11.88 extracted from
- Efficient biosynthesis of vanillin from isoeugenol by recombinant isoeugenol monooxygenase from Pseudomonas nitroreducens Jin1 (2021), Appl. Biochem. Biotechnol., 193, 1116-1128 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas nitroreducens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isoeugenol + O2 | Pseudomonas nitroreducens | - |
vanillin + acetaldehyde | - |
? |  |
| isoeugenol + O2 | Pseudomonas nitroreducens Jin1 | - |
vanillin + acetaldehyde | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | 10% (v/v) DMSO is the optimal co-solvent for reaction | Pseudomonas nitroreducens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas nitroreducens | C3VA26 | - |
- |
| Pseudomonas nitroreducens Jin1 | C3VA26 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isoeugenol + O2 | - |
Pseudomonas nitroreducens | vanillin + acetaldehyde | - |
? | |
| isoeugenol + O2 | the conversion is significantly increased at high substrate concentration (50 and 100 mM) | Pseudomonas nitroreducens | vanillin + acetaldehyde | - |
? | |
| isoeugenol + O2 | - |
Pseudomonas nitroreducens Jin1 | vanillin + acetaldehyde | - |
? | |
| isoeugenol + O2 | the conversion is significantly increased at high substrate concentration (50 and 100 mM) | Pseudomonas nitroreducens Jin1 | vanillin + acetaldehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| iem | - |
Pseudomonas nitroreducens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 20 | conversion after 12 h increases from 67.0 to 84.1% with the temperature increase from 15 to 20°C, and it decreases beyond 20°C | Pseudomonas nitroreducens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
the enzyme is relatively stable at pH 9.0, with a residual activity over 90% after 12 h | Pseudomonas nitroreducens |
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Release 2023.1 (January 2023)
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