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Literature summary for 1.13.11.88 extracted from

  • Zhao, L.; Jiang, Y.; Fang, H.; Zhang, H.; Cheng, S.; Rajoka, M.S.R.; Wu, Y.
    Biotransformation of isoeugenol into vanillin using immobilized recombinant cells containing isoeugenol monooxygenase active aggregates (2019), Appl. Biochem. Biotechnol., 189, 448-458 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase is efficiently expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

General Stability

General Stability Organism
the relative activity of the immobilized enzyme (with 100 mM glutaraldehyde) maintains over 60% after 7 recycles Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isoeugenol + O2 Pseudomonas putida
-
 vanillin + acetaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isoeugenol + O2
-
 Pseudomonas putida vanillin + acetaldehyde
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, recombinant IEM720-18A fusion protein, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
iem
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Pseudomonas putida