Application | Comment | Organism |
---|---|---|
synthesis | upon expression of mutant F281Q in Escherichia coli and employing sol-gel chitosan membrane for removing the produced vanillin from the biotransformation system, under the optimal conditions (pH 8.5, 30°C, 180 rpm, 0.5 g wet cells, 0.1 g chitosan membrane), vanillin concentrations reach 4.5 g/l after about 40 h | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure and docking of substrate isoeugenol to the active site | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
F281Q | mutant displays the highest conversion of isoeugenol | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.145 | - |
isoeugenol | wild-type, 30°C, pH not specified in the publication | Pseudomonas sp. | |
0.15 | - |
isoeugenol | mutant F281Q, 30°C, pH not specified in the publication | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | A0A384ZRB9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.6 | - |
wild-type, 30°C, pH not specified in the publication | Pseudomonas sp. |
7.5 | - |
mutant F281Q, 30°C, pH not specified in the publication | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isoeugenol + O2 | - |
Pseudomonas sp. | vanillin + acetaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, calculated from sequence | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
Iem720 | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.21 | - |
isoeugenol | wild-type, 30°C, pH not specified in the publication | Pseudomonas sp. | |
7.58 | - |
isoeugenol | mutant F281Q, 30°C, pH not specified in the publication | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas sp. |