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Literature summary for 1.13.11.88 extracted from

  • Zhao, L.; Xie, Y.; Chen, L.; Xu, X.; Zhao, C.; Cheng, F.
    Efficient biotransformation of isoeugenol to vanillin in recombinant strains of Escherichia coli by using engineered isoeugenol monooxygenase and sol-gel chitosan membrane (2018), Process Biochem., 71, 76-81 .
No PubMed abstract available

Application

Application Comment Organism
synthesis upon expression of mutant F281Q in Escherichia coli and employing sol-gel chitosan membrane for removing the produced vanillin from the biotransformation system, under the optimal conditions (pH 8.5, 30°C, 180 rpm, 0.5 g wet cells, 0.1 g chitosan membrane), vanillin concentrations reach 4.5 g/l after about 40 h Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure and docking of substrate isoeugenol to the active site Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
F281Q mutant displays the highest conversion of isoeugenol Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.145
-
isoeugenol wild-type, 30°C, pH not specified in the publication Pseudomonas sp.
0.15
-
isoeugenol mutant F281Q, 30°C, pH not specified in the publication Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. A0A384ZRB9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
wild-type, 30°C, pH not specified in the publication Pseudomonas sp.
7.5
-
mutant F281Q, 30°C, pH not specified in the publication Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isoeugenol + O2
-
Pseudomonas sp. vanillin + acetaldehyde
-
?

Subunits

Subunits Comment Organism
? x * 53000, calculated from sequence Pseudomonas sp.

Synonyms

Synonyms Comment Organism
Iem720
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.21
-
isoeugenol wild-type, 30°C, pH not specified in the publication Pseudomonas sp.
7.58
-
isoeugenol mutant F281Q, 30°C, pH not specified in the publication Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas sp.