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Literature summary for 1.13.11.88 extracted from

  • Yamada, M.; Okada, Y.; Yoshida, T.; Nagasawa, T.
    Purification, characterization and gene cloning of isoeugenol-degrading enzyme from Pseudomonas putida IE27 (2007), Arch. Microbiol., 187, 511-517 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 1 mM, 90% loss of activity Pseudomonas putida
Ag+ 1 mM, 73% loss of activity Pseudomonas putida
Cu2+ 1 mM, 56% loss of activity Pseudomonas putida
Hg2+ 1 mM, 87% loss of activity Pseudomonas putida
additional information not inhibitory: NAD+, NADH, NADP+, NADPH, FMN and FAD Pseudomonas putida
p-chloromercuribenzoic acid 1 mM, 44% loss of activity Pseudomonas putida
phenylhydrazine 1 mM, 95% loss of activity Pseudomonas putida
R-cycloserine 1 mM, 48% loss of activity Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.175
-
isoeugenol pH 7.0, 30°C Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isoeugenol + O2 Pseudomonas putida
-
vanillin + acetaldehyde
-
?
additional information Pseudomonas putida enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin ?
-
?
additional information Pseudomonas putida no substrates: eugenol, coniferyl alcohol, coniferyl aldehyde, ferulic acid, 3,4-dihydroxycinnamic acid, 3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, 4-hydroxycinnamic acid, 3-pyridinepropionic acid, alpha-methoxycinnamic acid, styrene, trans-beta-methylstyrene, trans-stilbene, trans-stilbene oxide, acrylic acid and trans-2-hexenoic acid ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida A5HV13
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.53
-
pH 7.0, 30°C Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methoxy-4-vinylphenol + O2 about 1% of the activity with isoeugenol Pseudomonas putida vanillin + formaldehyde
-
?
isoeugenol + O2
-
Pseudomonas putida vanillin + acetaldehyde
-
?
additional information enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin Pseudomonas putida ?
-
?
additional information no substrates: eugenol, coniferyl alcohol, coniferyl aldehyde, ferulic acid, 3,4-dihydroxycinnamic acid, 3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, 4-hydroxycinnamic acid, 3-pyridinepropionic acid, alpha-methoxycinnamic acid, styrene, trans-beta-methylstyrene, trans-stilbene, trans-stilbene oxide, acrylic acid and trans-2-hexenoic acid Pseudomonas putida ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE, 1 * 548080, calculated from sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
ISO
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.18
-
isoeugenol pH 7.0, 30°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10.5 20°C, stable for 30 min Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29.8
-
isoeugenol pH 7.0, 30°C Pseudomonas putida