Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 1 mM, 90% loss of activity | Pseudomonas putida | |
Ag+ | 1 mM, 73% loss of activity | Pseudomonas putida | |
Cu2+ | 1 mM, 56% loss of activity | Pseudomonas putida | |
Hg2+ | 1 mM, 87% loss of activity | Pseudomonas putida | |
additional information | not inhibitory: NAD+, NADH, NADP+, NADPH, FMN and FAD | Pseudomonas putida | |
p-chloromercuribenzoic acid | 1 mM, 44% loss of activity | Pseudomonas putida | |
phenylhydrazine | 1 mM, 95% loss of activity | Pseudomonas putida | |
R-cycloserine | 1 mM, 48% loss of activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.175 | - |
isoeugenol | pH 7.0, 30°C | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isoeugenol + O2 | Pseudomonas putida | - |
vanillin + acetaldehyde | - |
? | |
additional information | Pseudomonas putida | enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin | ? | - |
? | |
additional information | Pseudomonas putida | no substrates: eugenol, coniferyl alcohol, coniferyl aldehyde, ferulic acid, 3,4-dihydroxycinnamic acid, 3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, 4-hydroxycinnamic acid, 3-pyridinepropionic acid, alpha-methoxycinnamic acid, styrene, trans-beta-methylstyrene, trans-stilbene, trans-stilbene oxide, acrylic acid and trans-2-hexenoic acid | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | A5HV13 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.53 | - |
pH 7.0, 30°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methoxy-4-vinylphenol + O2 | about 1% of the activity with isoeugenol | Pseudomonas putida | vanillin + formaldehyde | - |
? | |
isoeugenol + O2 | - |
Pseudomonas putida | vanillin + acetaldehyde | - |
? | |
additional information | enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin | Pseudomonas putida | ? | - |
? | |
additional information | no substrates: eugenol, coniferyl alcohol, coniferyl aldehyde, ferulic acid, 3,4-dihydroxycinnamic acid, 3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, 4-hydroxycinnamic acid, 3-pyridinepropionic acid, alpha-methoxycinnamic acid, styrene, trans-beta-methylstyrene, trans-stilbene, trans-stilbene oxide, acrylic acid and trans-2-hexenoic acid | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE, 1 * 548080, calculated from sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ISO | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.18 | - |
isoeugenol | pH 7.0, 30°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | 20°C, stable for 30 min | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.8 | - |
isoeugenol | pH 7.0, 30°C | Pseudomonas putida |