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Literature summary for 1.13.11.87 extracted from

  • Andler, R.; Valdes, C.; Diaz-Barrera, A.; Steinbuechel, A.
    Biotransformation of poly(cis-1,4-isoprene) in a multiphase enzymatic reactor for continuous extraction of oligo-isoprenoid molecules (2020), New Biotechnol., 58, 10-16 .
    View publication on PubMed
Search for more literature for 1.13.11.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Gordonia polyisoprenivorans

Inhibitors

Inhibitors Comment Organism Structure
ethyl acetate the presence of ethyl acetate does not completely inhibit the activity of the enzyme Gordonia polyisoprenivorans
pentane the presence of pentane does not completely inhibit the activity of the enzyme Gordonia polyisoprenivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,4-polyisoprene + n O2 Gordonia polyisoprenivorans the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) ?
-
 ?
cis-1,4-polyisoprene + n O2 Gordonia polyisoprenivorans VH2 the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gordonia polyisoprenivorans H6N162
-
-
Gordonia polyisoprenivorans VH2 H6N162
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gordonia polyisoprenivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
 in 0.2 M Tris buffer with pentane, at pH 7.0 and 30°C Gordonia polyisoprenivorans
3.25
-
 in 0.2 M Tris buffer with ethyl acetate, at pH 7.0 and 30°C Gordonia polyisoprenivorans
4.66
-
 in 0.2 M Tris buffer, at pH 7.0 and 30°C Gordonia polyisoprenivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + n O2 the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) Gordonia polyisoprenivorans ?
-
 ?
cis-1,4-polyisoprene + n O2 the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) Gordonia polyisoprenivorans VH2 ?
-
 ?
cis-1,4-polyisoprene rubber particles + O2 the mass distribution of the degradation products differs depending on the extraction solvent. In the presence of pentane, at least 21 oligo(cis-1,4-isoprene) molecules are detected ranging from 304 Da (3 isoprene units) to 1664 Da (23 isoprene units), whereas after ethyl acetate extraction, only 8 oligo(cis-1,4-isoprene) molecules are identified ranging from 304 Da (3 isoprene units) to 780 Da (10 isoprene units) Gordonia polyisoprenivorans ?
-
 ?
cis-1,4-polyisoprene rubber particles + O2 the mass distribution of the degradation products differs depending on the extraction solvent. In the presence of pentane, at least 21 oligo(cis-1,4-isoprene) molecules are detected ranging from 304 Da (3 isoprene units) to 1664 Da (23 isoprene units), whereas after ethyl acetate extraction, only 8 oligo(cis-1,4-isoprene) molecules are identified ranging from 304 Da (3 isoprene units) to 780 Da (10 isoprene units) Gordonia polyisoprenivorans VH2 ?
-
 ?
polyisoprene latex milk + O2 polyisoprene latex milk with an average molecular weight Mn of the polyisoprene of 400,000 g/mol Gordonia polyisoprenivorans ?
-
 ?
polyisoprene latex milk + O2 polyisoprene latex milk with an average molecular weight Mn of the polyisoprene of 400,000 g/mol Gordonia polyisoprenivorans VH2 ?
-
 ?

Synonyms

Synonyms Comment Organism
latex clearing protein
-
 Gordonia polyisoprenivorans
Lcp1
-
 Gordonia polyisoprenivorans

Cofactor

Cofactor Comment Organism Structure
heme
-
 Gordonia polyisoprenivorans