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Literature summary for 1.13.11.87 extracted from
- Solimonas fluminis has an active latex-clearing protein (2019), Appl. Microbiol. Biotechnol., 103, 8229-8239 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 cells | Solimonas fluminis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Diethyl dithiocarbamate | almost complete inhibition (99%) at 1 mM | Solimonas fluminis |  |
| imidazole | about 50% residual activity at 1 mM | Solimonas fluminis | |
| Pyridine | about 70% residual activity at 1 mM | Solimonas fluminis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-1,4-polyisoprene + n O2 | Solimonas fluminis | the enzyme cleaves cis-1,4-polyisoprene to a mixture of C20 and higher oligoisoprenoids | ? | - |
? | |
| cis-1,4-polyisoprene + n O2 | Solimonas fluminis HR-BB | the enzyme cleaves cis-1,4-polyisoprene to a mixture of C20 and higher oligoisoprenoids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solimonas fluminis | - |
- |
- |
| Solimonas fluminis HR-BB | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Strep-Tactin XT column chromatography and Superdex 200 gel filtration | Solimonas fluminis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-1,4-polyisoprene + n O2 | the enzyme cleaves cis-1,4-polyisoprene to a mixture of C20 and higher oligoisoprenoids | Solimonas fluminis | ? | - |
? | |
| cis-1,4-polyisoprene + n O2 | the enzyme cleaves cis-1,4-polyisoprene to a mixture of C20 and higher oligoisoprenoids | Solimonas fluminis HR-BB | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Solimonas fluminis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| latex-clearing protein | - |
Solimonas fluminis |
| Lcp protein | - |
Solimonas fluminis |
| polyisoprene-cleaving rubber oxygenase | - |
Solimonas fluminis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | b-heme-containing protein with an oxidized, 5fold coordinated (open) heme centre | Solimonas fluminis | |
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Release 2023.1 (January 2023)
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