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Literature summary for 1.13.11.87 extracted from

  • Ilcu, L.; Roether, W.; Birke, J.; Brausemann, A.; Einsle, O.; Jendrossek, D.
    Structural and functional analysis of Latex clearing protein (Lcp) provides insight into the enzymatic cleavage of rubber (2017), Sci. Rep., 7, 6179 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.87

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp. K30

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour diffusion method Streptomyces sp. K30

Protein Variants

Protein Variants Comment Organism
K167A specific activity of polyisoprene oxidation is reduced to 20%, the product spectrum is unchanged Streptomyces sp. K30
K167H specific activity of polyisoprene oxidation is reduced to 12%, the product spectrum is unchanged Streptomyces sp. K30
R164A mutant enzyme contains the heme cofactor, but shows no detectable activity Streptomyces sp. K30
T168A mutant enzyme contains the heme cofactor, but shows only 2% residual activity compared to the wild type Streptomyces sp. K30

Organism

Organism UniProt Comment Textmining
Streptomyces sp. K30 Q3L8N0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp. K30

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + O2 the enzyme acts as an endo-type dioxygenase producing C20 and higher oligo-isoprenoids that differ in the number of isoprene units but have the same terminal functions, CHO-CH2- and -CH2-COCH3 Streptomyces sp. K30 ?
-
 ?

Synonyms

Synonyms Comment Organism
latex clearing protein
-
 Streptomyces sp. K30
Lcp
-
 Streptomyces sp. K30
rubber oxygenase
-
 Streptomyces sp. K30

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Streptomyces sp. K30

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Streptomyces sp. K30

Cofactor

Cofactor Comment Organism Structure
cytochrome b-type cytochrome. The haem group of LcpK30 is ligated to the polypeptide by a proximal histidine (His198) and by a lysine residue (Lys167) as the distal axial ligand Streptomyces sp. K30