Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.87 extracted from

  • Roether, W.; Birke, J.; Grond, S.; Beltran, J.M.; Jendrossek, D.
    Production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber (2017), Microb. Biotechnol., 10, 1426-1433 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.87

Application

Application Comment Organism
synthesis production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber. A method for the preparation of reactive oligo-isoprenoids that can be used to convert polyisoprene latex or rubber waste materials into value-added molecules, biofuels, polyurethanes or other polymers. Emulsions of polyisoprene (latex) are treated with RoxAXsp, RoxBXsp, LcpK30 or with combinations of the three proteins. The cleavage products are purified by solvent extraction and FPLC separation. All products have the same general structure with terminal functions (CHO-CH2- and -CH2-COCH3) but differ in the number of intact isoprene units in between Streptomyces sp. K30
synthesis production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber. A method for the preparation of reactive oligo-isoprenoids that can be used to convert polyisoprene latex or rubber waste materials into value-added molecules, biofuels, polyurethanes or other polymers. Emulsions of polyisoprene (latex) are treated with RoxAXsp, RoxBXsp, LcpK30 or with combinations of the three proteins. The cleavage products are purified by solvent extraction and FPLC separation. All products have the same general structure with terminal functions (CHO-CH2- and -CH2-COCH3) but differ in the number of intact isoprene units in between Xanthomonas sp. 35Y

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp. K30
expression in Escherichia coli Xanthomonas sp. 35Y

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Xanthomonas sp. 35Y
70000
-
 gel filtration Streptomyces sp. K30

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,4-polyisoprene + n O2 Streptomyces sp. K30 the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) ?
-
 ?
cis-1,4-polyisoprene + n O2 Xanthomonas sp. 35Y the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) ?
-
 ?
cis-1,4-polyisoprene + O2 Streptomyces sp. K30
-
 ?
-
 ?
cis-1,4-polyisoprene + O2 Xanthomonas sp. 35Y
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. K30
-
-
-
Streptomyces sp. K30 Q3L8N0
-
-
Xanthomonas sp. 35Y
-
-
-
Xanthomonas sp. 35Y A0A1S6Q8F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp. K30
-
 Xanthomonas sp. 35Y

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + n O2 the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) Streptomyces sp. K30 ?
-
 ?
cis-1,4-polyisoprene + n O2 the enzyme produces a mixture of oligo-isoprenoids (C20, C25, C30 and higher oligo-isoprenoids) Xanthomonas sp. 35Y ?
-
 ?
cis-1,4-polyisoprene + O2
-
 Streptomyces sp. K30 ?
-
 ?
cis-1,4-polyisoprene + O2
-
 Xanthomonas sp. 35Y ?
-
 ?

Synonyms

Synonyms Comment Organism
latex-clearing protein
-
 Streptomyces sp. K30
Lcp
-
 Streptomyces sp. K30
LcpK30
-
 Streptomyces sp. K30
RoxB
-
 Xanthomonas sp. 35Y
rubber oxygenase
-
 Xanthomonas sp. 35Y

Cofactor

Cofactor Comment Organism Structure
heme
-
 Xanthomonas sp. 35Y
heme c-type diheme dioxygenase Streptomyces sp. K30