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Literature summary for 1.13.11.87 extracted from

  • Watcharakul, S.; Roether, W.; Birke, J.; Umsakul, K.; Hodgson, B.; Jendrossek, D.
    Biochemical and spectroscopic characterization of purified Latex Clearing Protein (Lcp) from newly isolated rubber degrading Rhodococcus rhodochrous strain RPK1 reveals novel properties of Lcp (2016), BMC Microbiol., 16, 92 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.87

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodococcus rhodochrous

Inhibitors

Inhibitors Comment Organism Structure
Diethyl dithiocarbamate 82 % inhibition. No effect on the UV(vis) spectrum of the enzyme and this excludes a direct effect of the inhibitor at the heme site Rhodococcus rhodochrous
additional information carbon monoxide has no inhibitory effect on the polyisoprene cleavage Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42200
-
 mature protein, SDS-PAGE Rhodococcus rhodochrous
45200
-
 preprotein, SDS-PAGE Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous A0A165QBG6
-
-
Rhodococcus rhodochrous RPK1 A0A165QBG6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the preprotein has a MW of 45200 Da, the mature protein has MW of 42200 Da Rhodococcus rhodochrous

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus rhodochrous

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
 23°C, pH 8.0 Rhodococcus rhodochrous
3.1
-
 30°C, pH 8.0 Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(cis-1,4-isoprene) + O2 the enzyme degrades poly(1,4-cis-isoprene) to a mixture of oligoisoprene molecules with terminal keto and aldehyde groups Rhodococcus rhodochrous ?
-
 ?
poly(cis-1,4-isoprene) + O2 the enzyme degrades poly(1,4-cis-isoprene) to a mixture of oligoisoprene molecules with terminal keto and aldehyde groups Rhodococcus rhodochrous RPK1 ?
-
 ?

Synonyms

Synonyms Comment Organism
latex clearing protein
-
 Rhodococcus rhodochrous
Lcp
-
 Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rhodococcus rhodochrous

Cofactor

Cofactor Comment Organism Structure
heme presence of one b-type haem in LcpRr as a co-factor is confirmed by metal analysis, solvent extraction, bipyridyl assay and detection of haem-b specific m/z values via mass-spectrometry Rhodococcus rhodochrous