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Literature summary for 1.13.11.87 extracted from

  • Birke, J.; Roether, W.; Jendrossek, D.
    Rhizobacter gummiphilus NS21 has two rubber oxygenases (RoxA and RoxB) acting synergistically in rubber utilisation (2018), Appl. Microbiol. Biotechnol., 102, 10245-10257 .
    View publication on PubMed
Search for more literature for 1.13.11.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a DELTAroxA35Y/DELTAroxB35Y background of Steroidobacter cummioxidans 35Y Rhizobacter gummiphilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70800
-
 mature protein, SDS-PAGE Rhizobacter gummiphilus
74000
-
 preprotein, SDS-PAGE Rhizobacter gummiphilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,4-polyisoprene + O2 Rhizobacter gummiphilus Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal ?
-
 ?
cis-1,4-polyisoprene + O2 Rhizobacter gummiphilus NS21 Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizobacter gummiphilus
-
-
-
Rhizobacter gummiphilus NS21
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhizobacter gummiphilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8
-
 23°C, pH not specified in the publication Rhizobacter gummiphilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + O2
-
 Rhizobacter gummiphilus ?
-
 ?
cis-1,4-polyisoprene + O2 Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal Rhizobacter gummiphilus ?
-
 ?
cis-1,4-polyisoprene + O2
-
 Rhizobacter gummiphilus NS21 ?
-
 ?
cis-1,4-polyisoprene + O2 Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal Rhizobacter gummiphilus NS21 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70800, mature protein, SDS-PAGE Rhizobacter gummiphilus

Synonyms

Synonyms Comment Organism
RoxB
-
 Rhizobacter gummiphilus

General Information

General Information Comment Organism
malfunction Gram-negative rubber-degrading bacteria generally utilise two synergistically acting rubber oxygenases (RoxA/RoxB) for efficient cleavage of polyisoprene to (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal Rhizobacter gummiphilus