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Literature summary for 1.13.11.87 extracted from

  • Birke, J.; Roether, W.; Jendrossek, D.
    RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp) (2017), Appl. Environ. Microbiol., 83, e00721-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.87

Cloned(Commentary)

Cloned (Comment) Organism
-
 Xanthomonas sp. 35Y

Inhibitors

Inhibitors Comment Organism Structure
CO oxygen consumption by RoxB is inhibited when carbon monoxide is added to an ongoing polyisoprene cleavage reaction Xanthomonas sp. 35Y
diethyldithiocarbamate
-
 Xanthomonas sp. 35Y
imidazole binding of this compounds to the active heme site inhibits the enzyme Xanthomonas sp. 35Y
additional information the use of 1 or 10 mM EDTA has no effect on the rubber-cleaving activity of RoxB Xanthomonas sp. 35Y
Pyridine binding of this compounds to the active heme site inhibits the enzyme Xanthomonas sp. 35Y
SDS
-
 Xanthomonas sp. 35Y

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70300
-
-
 Xanthomonas sp. 35Y

Organism

Organism UniProt Comment Textmining
Xanthomonas sp. 35Y A0A1S6Q8F9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the preprotein has a MW of 73800 Da, the mature protein has MW of 71500 Da Xanthomonas sp. 35Y

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthomonas sp. 35Y

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 23°C, pH and temperature not specified in the publication Xanthomonas sp. 35Y
5.7
-
 30°C, pH and temperature not specified in the publication Xanthomonas sp. 35Y
6.4
-
 37°C, pH and temperature not specified in the publication Xanthomonas sp. 35Y

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(cis-1,4-isoprene) + O2 the enzyme cleaves cis-1,4-polyisoprene polymers into a mixture of C20, C25, and higher oligo-isoprenoids with terminal aldehyde and keto groups were identified Xanthomonas sp. 35Y (4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal + (4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal + (4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal + ?
-
 ?

Synonyms

Synonyms Comment Organism
RoxB
-
 Xanthomonas sp. 35Y

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Xanthomonas sp. 35Y

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 80% of maximal activity, pH 9.0: about 70% of maximal activity Xanthomonas sp. 35Y

Cofactor

Cofactor Comment Organism Structure
heme 2 c-type hemes Xanthomonas sp. 35Y
heme 2 c-type hemes. Both heme sites of RoxB are present in an oxidized Fe3+ form Xanthomonas sp. 35Y

Expression

Organism Comment Expression
Xanthomonas sp. 35Y expression of the roxA gene is upregulated during growth on polyisoprene up

General Information

General Information Comment Organism
metabolism the synergistic effect of the simultaneous presence of RoxA35Y and RoxB35Y enables the organism to convert rubber extracellularly at high efficiency to just one cleavage product of defined length ((4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal) that can be taken up by only one transport protein and can be used as a source of carbon and energy Xanthomonas sp. 35Y