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Literature summary for 1.13.11.87 extracted from

  • Birke, J.; Jendrossek, D.
    Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms (2014), Appl. Environ. Microbiol., 80, 5012-5020 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.87

Cloned(Commentary)

Cloned (Comment) Organism
expression of Lcp of Streptomyces sp. K30 in a DELTAroxA background of Xanthomonas sp. strain 35Y Streptomyces sp. K30

Inhibitors

Inhibitors Comment Organism Structure
alpha-tocopherol 1 mM, 40% inhibition Streptomyces sp. K30
ascorbate 1 mM, 20% inhibition Streptomyces sp. K30
carotene 1 mM, 40% inhibition Streptomyces sp. K30
diethyldithiocarbamate 1 mM, 50% inhibition Streptomyces sp. K30
dithiothreitol 10 mM, 90% inhibition Streptomyces sp. K30
EDTA 1 mM, 30% inhibition Streptomyces sp. K30
ethanol 5%, 10% inhibition Streptomyces sp. K30
imidazole inhibits by 35% and 85% at 1 mM and 10 mM Streptomyces sp. K30
Phenanthroline 1 mM, 10% inhibition Streptomyces sp. K30
SDS 0.1%, complete inhibition Streptomyces sp. K30
squalene 1 mM, 10% inhibition Streptomyces sp. K30

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp. K30
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no indication for the presence of iron, other metals, or cofactors in Lcp Streptomyces sp. K30

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Streptomyces sp. K30

Organism

Organism UniProt Comment Textmining
Streptomyces sp. K30 Q3L8N0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp. K30

Storage Stability

Storage Stability Organism
almost stable during storage on ice Streptomyces sp. K30

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + O2 Lcp degrades polyisoprene via endo-type cleavage to tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups Streptomyces sp. K30 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Streptomyces sp. K30

Synonyms

Synonyms Comment Organism
latex-clearing protein
-
 Streptomyces sp. K30
Lcp
-
 Streptomyces sp. K30

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
23
-
 Lcp activity decreases by incubation at 23°C by 70% within the 22 h of incubation time Streptomyces sp. K30
37
-
 Lcp activity decreases by incubation at 37°C by 80% within the 22 h of incubation time Streptomyces sp. K30

Cofactor

Cofactor Comment Organism Structure
additional information no indication for the presence of iron, other metals, or cofactors in Lcp Streptomyces sp. K30