Literature summary for 1.13.11.87 extracted from

Braaz, R.; Armbruster, W.; Jendrossek, D.
Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-Isoprene) by a dioxygenase mechanism (2005), Appl. Environ. Microbiol., 71, 2473-2478 .

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Organism

Organism	UniProt	Comment	Textmining
Xanthomonas sp. 35Y	Q7X0P3	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Xanthomonas sp. 35Y

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
poly(cis-1,4-isoprene) + O2	12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is the main cleavage product in the absence of (18)O-compounds. Incorporation of one (18)O atom in 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is found if the cleavage reaction is performed in the presence of (18)O2 and H2(16)O. Incubation of poly(cis-1,4-isoprene) (with RoxA) or of isolated unlabeled 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (without RoxA) with H2(18)O in the presence of (16)O2 indicates that the carbonyl oxygen atoms of 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is significantly exchanged with oxygen atoms derived from water. The isotope exchange is avoided by simultaneous enzymatic reduction of both carbonyl functions of 12-Oxo-4,8-dimethyltrideca-4,8-diene-1-al to the corresponding dialcohol 12-hydroxy-4,8-dimethyltrideca-4,8-diene-1-ol during RoxA-mediated in vitro cleavage of poly(cis-1,4-isoprene)	Xanthomonas sp. 35Y	(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal	-	?
rubber latex + O2	RoxA cleaves rubber by a dioxygenase mechanism	Xanthomonas sp. 35Y	(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal	-	?

Synonyms

Synonyms	Comment	Organism
roxA	-	Xanthomonas sp. 35Y

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	assay at	Xanthomonas sp. 35Y

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Xanthomonas sp. 35Y

Cofactor

Cofactor	Comment	Organism	Structure
heme	the enzyme contains 2 mol of heme per mol of RoxA	Xanthomonas sp. 35Y

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Release 2023.1 (January 2023)