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Literature summary for 1.13.11.85 extracted from

  • Braaz, R.; Armbruster, W.; Jendrossek, D.
    Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-Isoprene) by a dioxygenase mechanism (2005), Appl. Environ. Microbiol., 71, 2473-2478 .
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Xanthomonas sp. 35Y Q7X0P3
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-

Purification (Commentary)

Purification (Comment) Organism
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Xanthomonas sp. 35Y

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + n O2
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Xanthomonas sp. 35Y n 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al
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?
poly(cis-1,4-isoprene) + O2 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is the main cleavage product in the absence of (18)O-compounds. Incorporation of one (18)O atom in 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is found if the cleavage reaction is performed in the presence of (18)O2 and H2(16)O. Incubation of poly(cis-1,4-isoprene) (with RoxA) or of isolated unlabeled 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (without RoxA) with H2(18)O in the presence of (16)O2 indicates that the carbonyl oxygen atoms of 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al is significantly exchanged with oxygen atoms derived from water. The isotope exchange is avoided by simultaneous enzymatic reduction of both carbonyl functions of 12-Oxo-4,8-dimethyltrideca-4,8-diene-1-al to the corresponding dialcohol 12-hydroxy-4,8-dimethyltrideca-4,8-diene-1-ol during RoxA-mediated in vitro cleavage of poly(cis-1,4-isoprene) Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
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?
rubber latex + O2 RoxA cleaves rubber by a dioxygenase mechanism Xanthomonas sp. 35Y (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
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?

Synonyms

Synonyms Comment Organism
roxA
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Xanthomonas sp. 35Y

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
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assay at Xanthomonas sp. 35Y

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
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assay at Xanthomonas sp. 35Y

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains 2 mol of heme per mol of RoxA Xanthomonas sp. 35Y