Cloned (Comment) | Organism |
---|---|
- |
Streptomyces toyocaensis |
Crystallization (Comment) | Organism |
---|---|
structure of the enzyme in complex with a bound substrate mimic, crystallized by the hanging drop method at 20°C, 2.75 A resolution | Streptomyces toyocaensis |
Protein Variants | Comment | Organism |
---|---|---|
L237T | kcat/KM for (3,5-dihydroxyphenyl)acetyl-CoA is 2% compared to the wild-type activity | Streptomyces toyocaensis |
V425T | kcat/KM for (3,5-dihydroxyphenyl)acetyl-CoA is 6% compared to the wild-type activity | Streptomyces toyocaensis |
V429T | kcat/KM for (3,5-dihydroxyphenyl)acetyl-CoA is 3% compared to the wild-type activity | Streptomyces toyocaensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]methyl (3R)-4-([3-[(2-[[(2,5-dihydroxyphenyl)acetyl]amino]ethyl)amino]-3-oxopropyl]amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl dihydrogen diphosphate | - |
Streptomyces toyocaensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
O2 | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.0021 | - |
O2 | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.0026 | - |
O2 | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.0039 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.0138 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.0147 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.058 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not dependent on a metal ion | Streptomyces toyocaensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
354000 | - |
gel filtration | Streptomyces toyocaensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | Streptomyces toyocaensis | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | Streptomyces toyocaensis A47934 | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces toyocaensis | Q8KLK7 | - |
- |
Streptomyces toyocaensis A47934 | Q8KLK7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces toyocaensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(3,5-dihydroxyphenyl)acetyl-CoA + O2 = 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA | proposed catalytic mechanism | Streptomyces toyocaensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis A47934 | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis A47934 | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | crystallographic data | Streptomyces toyocaensis |
Synonyms | Comment | Organism |
---|---|---|
DpgC | - |
Streptomyces toyocaensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Streptomyces toyocaensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.037 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.05 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
0.172 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces toyocaensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor detected | Streptomyces toyocaensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]methyl (3R)-4-([3-[(2-[[(2,5-dihydroxyphenyl)acetyl]amino]ethyl)amino]-3-oxopropyl]amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl dihydrogen diphosphate | pH 7.4, 24°C | Streptomyces toyocaensis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics | Streptomyces toyocaensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.86 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
1.3 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
2.7 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
44 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis |