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Literature summary for 1.13.11.8 extracted from
- Protocatechuate 4,5-dioxygenase from Pseudomonas testosteroni (1990), Methods Enzymol., 188, 89-95.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Comamonas testosteroni |
General Stability
| General Stability | Organism |
|---|---|
| dialysis can be performed only at temperatures at or below 4°C | Comamonas testosteroni |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
protocatechuate | - |
Comamonas testosteroni |  |
| 0.125 | - |
O2 | - |
Comamonas testosteroni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | present at a significant mol fraction, metal-free enzyme is inactive, iron can be removed by treatment with tiron | Comamonas testosteroni | |
| Fe2+ | iron is present in active enzyme in the high-spin ferrous state | Comamonas testosteroni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17700 | - |
alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE | Comamonas testosteroni |
| 33800 | - |
alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE | Comamonas testosteroni |
| 142000 | - |
gel filtration | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protocatechuate + O2 | Comamonas testosteroni | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
| protocatechuate + O2 | Comamonas testosteroni Pt-L5 / ATCC 49249 | - |
4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | - |
- |
- |
| Comamonas testosteroni | - |
grown on p-hydroxybenzoate-containing medium | - |
| Comamonas testosteroni Pt-L5 / ATCC 49249 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| rapidly inactivated by oxidizing agents such as H2O2 or ferricyanide, partial reactivation by ascorbate | Comamonas testosteroni |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 212 | - |
- |
Comamonas testosteroni |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -196°C, in liquid nitrogen, without significant loss of activity for several years | Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methylprotocatechuate + O2 | - |
Comamonas testosteroni | 4-carboxy-2-hydroxy-3-methylmuconate semialdehyde | - |
? | |
| 5-methylprotocatechuate + O2 | - |
Comamonas testosteroni Pt-L5 / ATCC 49249 | 4-carboxy-2-hydroxy-3-methylmuconate semialdehyde | - |
? | |
| protocatechuate + O2 | - |
Comamonas testosteroni | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
| protocatechuate + O2 | - |
Comamonas testosteroni Pt-L5 / ATCC 49249 | 4-carboxy-2-hydroxymuconate semialdehyde | - |
? | |
| sulfonylcatechol + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| sulfonylcatechol + O2 | - |
Comamonas testosteroni Pt-L5 / ATCC 49249 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2, beta2. 2 * 17700 + 2 * 33800, dimer of a heterodimer, SDS-PAGE | Comamonas testosteroni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 350 | - |
protocatechuate | - |
Comamonas testosteroni | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Comamonas testosteroni |
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