Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | activates by 27% at 5 mM | Saccharomyces cerevisiae | |
Triton X-100 | activates by 7% at 1% w/v | Saccharomyces cerevisiae | |
Tween 20 | activates by 3% at 1% w/v | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
synthesis | enzyme ScBCO2 potentially can be used for the enzymatic biotransformation of beta-carotene to beta-apo-10'-carotenal in biotechnological applications | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits by 10% at 5 mM | Saccharomyces cerevisiae | |
Fe2+ | inhibits by 37% at 5 mM | Saccharomyces cerevisiae | |
Mn2+ | inhibits by 13% at 5 mM | Saccharomyces cerevisiae | |
SDS | 99% inhibition at 1 % | Saccharomyces cerevisiae | |
Urea | 86% inhibition at 4 M | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
all-trans-beta-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
0.048 | - |
alpha-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
0.105 | - |
lutein | pH 8.0, 45°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates by 26% at 5 mM | Saccharomyces cerevisiae | |
Cu2+ | activates by 23% at 5 mM | Saccharomyces cerevisiae | |
Mg2+ | activates by 14% at 5 mM | Saccharomyces cerevisiae | |
Ni2+ | activates by 7% at 5 mM | Saccharomyces cerevisiae | |
Zn2+ | activates by 17% at 5 mM | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-beta-carotene + O2 | Saccharomyces cerevisiae | - |
all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
all-trans-beta-carotene + O2 | Saccharomyces cerevisiae ULI3 | - |
all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
alpha-carotene + O2 | Saccharomyces cerevisiae | - |
beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
alpha-carotene + O2 | Saccharomyces cerevisiae ULI3 | - |
beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
lutein + O2 | Saccharomyces cerevisiae | - |
? | - |
? | |
lutein + O2 | Saccharomyces cerevisiae ULI3 | - |
? | - |
? | |
additional information | Saccharomyces cerevisiae | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | ? | - |
? | |
additional information | Saccharomyces cerevisiae ULI3 | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
2-butanone | inhibits 66% at 50 v/v | Saccharomyces cerevisiae |
Acetone | inhibits 20% at 50 v/v | Saccharomyces cerevisiae |
benzene | inhibits 28% at 50 v/v | Saccharomyces cerevisiae |
chloroform | inhibits 57% at 50 v/v | Saccharomyces cerevisiae |
dichloromethane | inhibits 49% at 50 v/v | Saccharomyces cerevisiae |
dimethylformamide | inhibits 24% at 50 v/v | Saccharomyces cerevisiae |
DMSO | inhibits 17% at 50 v/v | Saccharomyces cerevisiae |
toluene | inhibits 28% at 50 v/v | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae ULI3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 9.5fold to homogeneity by ammonium sulfate fractionation and gel filtration | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.38 | - |
purified native enzyme, pH 8.0, 45°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-beta-carotene + O2 | - |
Saccharomyces cerevisiae | all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
all-trans-beta-carotene + O2 | - |
Saccharomyces cerevisiae ULI3 | all-trans-10'-apo-beta-carotenal + beta-ionone | - |
? | |
alpha-carotene + O2 | - |
Saccharomyces cerevisiae | beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
alpha-carotene + O2 | - |
Saccharomyces cerevisiae ULI3 | beta-apo-10'-carotenal + alpha-apo-10'-carotenal | - |
? | |
lutein + O2 | - |
Saccharomyces cerevisiae | ? | - |
? | |
lutein + O2 | - |
Saccharomyces cerevisiae ULI3 | ? | - |
? | |
additional information | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | Saccharomyces cerevisiae | ? | - |
? | |
additional information | enzyme ScBCO2 only exhibits cleavage activity towards carotenoid substrates containing two beta-ionone rings and its catalytic efficiency (kcat/Km) follows the descending order beta-carotene, alpha-carotene, lutein | Saccharomyces cerevisiae | ? | - |
? | |
additional information | ScBCO2-catalyzed conversion of beta-carotene to beta-apo-10'-carotenal is followed by HPLC and LC-MS | Saccharomyces cerevisiae ULI3 | ? | - |
? | |
additional information | enzyme ScBCO2 only exhibits cleavage activity towards carotenoid substrates containing two beta-ionone rings and its catalytic efficiency (kcat/Km) follows the descending order beta-carotene, alpha-carotene, lutein | Saccharomyces cerevisiae ULI3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
beta-carotene-9,10'-oxygenase | - |
Saccharomyces cerevisiae |
ScBCO2 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | activity range, profile overview | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | - |
lutein | pH 8.0, 45°C | Saccharomyces cerevisiae | |
0.0392 | - |
alpha-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
0.0475 | - |
all-trans-beta-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 50 % of the maximal activity at pH 7.0 and pH 9.0, 20% at pH 6.0 and pH 9.5 | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme converts beta-carotene to beta-apo-10'-carotenal, which is a precursor of the plant hormone strigolactone | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.159 | - |
lutein | pH 8.0, 45°C | Saccharomyces cerevisiae | |
0.817 | - |
alpha-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae | |
1.284 | - |
all-trans-beta-carotene | pH 8.0, 45°C | Saccharomyces cerevisiae |