BRENDA - Enzyme Database
show all sequences of 1.13.11.69

Insights into the formation of carlactone from in-depth analysis of the CCD8-catalyzed reactions

Bruno, M.; Vermathen, M.; Alder, A.; Wuest, F.; Schaub, P.; van der Steen, R.; Beyer, P.; Ghisla, S.; Al-Babili, S.; FEBS Lett. 591, 792-800 (2017)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
involved in catalysis, mechanism overview
Pisum sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
9-cis-10'-apo-beta-carotenal + 2 O2
Pisum sativum
-
carlactone + (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pisum sativum
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
9-cis-10'-apo-beta-carotenal + 2 O2 = carlactone + (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal
proposed mechanism of 9-cis-beta-apo-10'-carotenal conversion into carlactone, overview
Pisum sativum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
9-cis-10'-apo-beta-carotenal + 2 O2
-
744912
Pisum sativum
carlactone + (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
carotenoid cleavage dioxygenase 8
-
Pisum sativum
CCD8
-
Pisum sativum
PsCCD8
-
Pisum sativum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
involved in catalysis, mechanism overview
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
9-cis-10'-apo-beta-carotenal + 2 O2
Pisum sativum
-
carlactone + (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
9-cis-10'-apo-beta-carotenal + 2 O2
-
744912
Pisum sativum
carlactone + (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
the carotenoid cleavage dioxygenase 8, CCD8, catalyzes a series of reactions and molecular rearrangements for biosynthesis of strigolactones, a class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor 9-cis-beta-apo-10'-carotenal, a cis-configured beta-carotene cleavage product. Carlactone and (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal, and reaction intermediates structure analysis by LC-MS and NMR, overview
Pisum sativum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the carotenoid cleavage dioxygenase 8, CCD8, catalyzes a series of reactions and molecular rearrangements for biosynthesis of strigolactones, a class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor 9-cis-beta-apo-10'-carotenal, a cis-configured beta-carotene cleavage product. Carlactone and (2E,4E,6E)-7-hydroxy-4-methylhepta-2,4,6-trienal, and reaction intermediates structure analysis by LC-MS and NMR, overview
Pisum sativum
Other publictions for EC 1.13.11.69
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744912
Bruno
Insights into the formation o ...
Pisum sativum
FEBS Lett.
591
792-800
2017
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744876
Harrison
Biochemical characterization ...
Arabidopsis thaliana
FEBS J.
282
3986-4000
2015
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720753
Guan
Diverse roles of strigolactone ...
Zea mays
Plant Physiol.
160
1303-1317
2012
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721057
Alder
The path from beta-carotene to ...
Pisum sativum, Arabidopsis thaliana
Science
335
1348-1351
2012
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728380
Kohlen
The tomato CAROTENOID CLEAVAGE ...
Solanum lycopersicum
New Phytol.
196
535-547
2012
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718523
Jiang
-
MAX4 gene is involved in the r ...
Arabidopsis thaliana
Acta Physiol. Plant.
33
867-875
2011
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720559
Ledger
Modified CAROTENOID CLEAVAGE D ...
Actinidia chinensis
New Phytol.
188
803-813
2010
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720702
Auldridge
Characterization of three memb ...
Arabidopsis thaliana
Plant J.
45
982-993
2006
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720701
Bainbridge
Hormonally controlled expressi ...
Arabidopsis thaliana
Plant J.
44
569-580
2005
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719823
Schwartz
The biochemical characterizati ...
Arabidopsis thaliana
J. Biol. Chem.
279
46940-46945
2004
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719552
Sorefan
MAX4 and RMS1 are orthologous ...
Arabidopsis thaliana
Genes Dev.
17
1469-1474
2003
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