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Literature summary for 1.13.11.66 extracted from

  • Kolvenbach, B.A.; Lenz, M.; Benndorf, D.; Rapp, E.; Fousek, J.; Vlcek, C.; Schaeffer, A.; Gabriel, F.L.; Kohler, H.P.; Corvini, P.F.
    Purification and characterization of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3 (2011), AMB Express, 1, 08.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.66

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxybenzoate 0.2 mM, 94% inhibition; 0.2 mM, 94% inhibition Sphingomonas sp.
4-coumaric acid 0.2 mM, 97% inhibition; 0.2 mM, 97% inhibition Sphingomonas sp.
4-hydroxybenzoate 0.2 mM, 46% inhibition; 0.2 mM, 46% inhibition Sphingomonas sp.
caffeic acid 0.2 mM, 98% inhibition; 0.2 mM, 98% inhibition Sphingomonas sp.
catechol 0.2 mM, 93% inhibition; 0.2 mM, 93% inhibition Sphingomonas sp.
phenol 0.2 mM, 98% inhibition; 0.2 mM, 98% inhibition Sphingomonas sp.
resorcinol 0.2 mM, 99% inhibition; 0.2 mM, 99% inhibition Sphingomonas sp.
vanillate 0.2 mM, 86% inhibition; 0.2 mM, 86% inhibition Sphingomonas sp.
Vanillyl alcohol 0.2 mM, 62% inhibition; 0.2 mM, 62% inhibition Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 hydroquinone pH 7.0, 25°C Sphingomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 1.4 mol of Fe2+ per mol of enzyme Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.
38000
-
 2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.
120000
-
 gel filtration Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. F8TW82 small subunit
-
Sphingomonas sp. F8TW83 large subunit
-
Sphingomonas sp. TTNP3 F8TW82 small subunit
-
Sphingomonas sp. TTNP3 F8TW83 large subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1
-
 pH 7.0, 25°C Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(1-methyl1-octyl)-hydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-(1-methyl1-octyl)-hydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
2-chlorohydroquinone + O2 29% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-chlorohydroquinone + O2 29% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
2-ethylhydroquinone + O2 83% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-ethylhydroquinone + O2 83% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
2-hexylhydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-hexylhydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
2-methoxyhydroquinone + O2 59% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-methylhydroquinone + O2 139% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-pentylhydroquinone + O2 19% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-propylhydroquinone + O2 23% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
2-tert-butylhydroquinone + O2 5% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
hydroquinone + O2 consumption of an equimolar amount of molecular oxygen Sphingomonas sp. 4-hydroxymuconic acid semialdehyde
-
 ?
additional information enzyme catalyzes the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydroquinones Sphingomonas sp. ?
-
 ?
additional information enzyme catalyzes the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydroquinones Sphingomonas sp. TTNP3 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.5
-
 hydroquinone pH 7.0, 25°C Sphingomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
 Sphingomonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6136
-
 hydroquinone pH 7.0, 25°C Sphingomonas sp.