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Literature summary for 1.13.11.66 extracted from

  • Kolvenbach, B.A.; Lenz, M.; Benndorf, D.; Rapp, E.; Fousek, J.; Vlcek, C.; Schaeffer, A.; Gabriel, F.L.; Kohler, H.P.; Corvini, P.F.
    Purification and characterization of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3 (2011), AMB Express, 1, 08.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxybenzoate 0.2 mM, 94% inhibition; 0.2 mM, 94% inhibition Sphingomonas sp.
4-coumaric acid 0.2 mM, 97% inhibition; 0.2 mM, 97% inhibition Sphingomonas sp.
4-hydroxybenzoate 0.2 mM, 46% inhibition; 0.2 mM, 46% inhibition Sphingomonas sp.
caffeic acid 0.2 mM, 98% inhibition; 0.2 mM, 98% inhibition Sphingomonas sp.
catechol 0.2 mM, 93% inhibition; 0.2 mM, 93% inhibition Sphingomonas sp.
phenol 0.2 mM, 98% inhibition; 0.2 mM, 98% inhibition Sphingomonas sp.
resorcinol 0.2 mM, 99% inhibition; 0.2 mM, 99% inhibition Sphingomonas sp.
vanillate 0.2 mM, 86% inhibition; 0.2 mM, 86% inhibition Sphingomonas sp.
Vanillyl alcohol 0.2 mM, 62% inhibition; 0.2 mM, 62% inhibition Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
hydroquinone pH 7.0, 25°C Sphingomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 1.4 mol of Fe2+ per mol of enzyme Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.
38000
-
2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.
120000
-
gel filtration Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. F8TW82 small subunit
-
Sphingomonas sp. F8TW83 large subunit
-
Sphingomonas sp. TTNP3 F8TW82 small subunit
-
Sphingomonas sp. TTNP3 F8TW83 large subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
Sphingomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1
-
pH 7.0, 25°C Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(1-methyl1-octyl)-hydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-(1-methyl1-octyl)-hydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
?
2-chlorohydroquinone + O2 29% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-chlorohydroquinone + O2 29% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
?
2-ethylhydroquinone + O2 83% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-ethylhydroquinone + O2 83% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
?
2-hexylhydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-hexylhydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
?
2-methoxyhydroquinone + O2 59% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-methylhydroquinone + O2 139% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-pentylhydroquinone + O2 19% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-propylhydroquinone + O2 23% of the activity with hydroquinone Sphingomonas sp. ?
-
?
2-tert-butylhydroquinone + O2 5% of the activity with hydroquinone Sphingomonas sp. ?
-
?
hydroquinone + O2 consumption of an equimolar amount of molecular oxygen Sphingomonas sp. 4-hydroxymuconic acid semialdehyde
-
?
additional information enzyme catalyzes the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydroquinones Sphingomonas sp. ?
-
?
additional information enzyme catalyzes the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydroquinones Sphingomonas sp. TTNP3 ?
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.5
-
hydroquinone pH 7.0, 25°C Sphingomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
Sphingomonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6136
-
hydroquinone pH 7.0, 25°C Sphingomonas sp.