Cloned (Comment) | Organism |
---|---|
gene BCMO1, overlapping binding sites for HNF-1alpha and HNF-4alpha in the human BCMO1 gene proximal promoter. Endogenous nuclear HNF-1alpha and HNF-4alpha proteins competitively bind these sites in Caco-2 BBe cells. BCMO1 gene expression levels and promoter activity are significantly decreased in HNF-1alpha siRNA-transfected Caco-2 BBe cells. In contrast, HNF-4alpha siRNA-transfected cells exhibit a significant increase in BCMO1 gene expression and promoter activity. Mutation of these overlapping binding sites dramatically decreases BCMO1 promoter activity. Quantitative real-time reverse transcription-PCR enzyme expression analysis and BCMO1 promoter activity analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | Homo sapiens | - |
2 all-trans-retinal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HAY6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Caco-2 BBe cell | - |
Homo sapiens | - |
intestine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | - |
Homo sapiens | 2 all-trans-retinal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bcmo1 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | endogenous nuclear HNF-1alpha and HNF-4alpha proteins competitively bind these sites in Caco-2 BBe cells. BCMO1 gene expression levels and promoter activity are significantly decreased in HNF-1alpha siRNA-transfected Caco-2 BBe cells. In contrast, HNF-4alpha siRNA-transfected cells exhibit a significant increase in BCMO1 gene expression and promoter activity. Mutation of these overlapping binding sites dramatically decreases BCMO1 promoter activity | down |
Homo sapiens | endogenous nuclear HNF-1alpha and HNF-4alpha proteins competitively bind these sites in Caco-2 BBe cells. BCMO1 gene expression levels and promoter activity are significantly decreased in HNF-1alpha siRNA-transfected Caco-2 BBe cells. In contrast, HNF-4alpha siRNA-transfected cells exhibit a significant increase in BCMO1 gene expression and promoter activity. Mutation of these overlapping binding sites dramatically decreases BCMO1 promoter activity | up |
General Information | Comment | Organism |
---|---|---|
physiological function | competitive regulation of human intestinal beta-carotene 15,15'-monooxygenase 1 (BCMO1) gene expression by hepatocyte nuclear factor (HNF)-1alpha and HNF-4alpha, which are liver-enriched transcription factors that are also expressed in the small intestine, overview | Homo sapiens |