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Literature summary for 1.13.11.63 extracted from

  • Kim, N.; Kim, Y.; Kim, H.; Oh, D.
    Optimized formation of detergent micelles of beta-carotene and retinal production using recombinant human beta, beta-carotene 15,15-monooxygenase (2008), Biotechnol. Prog., 24, 227-231.
    View publication on PubMed
Search for more literature for 1.13.11.63

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET-24b+ for expression in Escherichia coli ER2566 cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 determined by SDS-PAGE Homo sapiens
63400
-
 theoretical, His-tagged protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + O2 Homo sapiens
-
 retinal
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAY6
-
-

Purification (Commentary)

Purification (Comment) Organism
using a His-Trap HP affinity chromatography column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.97
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2
-
 Homo sapiens retinal
-
 ?

Synonyms

Synonyms Comment Organism
beta,beta-carotene 15,15'-monooxygenase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Homo sapiens