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Literature summary for 1.13.11.61 extracted from

  • Andreou, A.Z.; Vanko, M.; Bezakova, L.; Feussner, I.
    Properties of a mini 9R-lipoxygenase from Nostoc sp. PCC 7120 and its mutant forms (2008), Phytochemistry, 69, 1832-1837.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant carboxy-terminal domain is purified after expression in Escherichia coli Nostoc sp.

Protein Variants

Protein Variants Comment Organism
A162G the recombinant carboxy-terminal domain of the wild-type enzyme and of mutant enzyme A162G produce primarily (9R,10EZ,12Z)-9-hydroperoxy-10,12-octadecadienoate from linoleate. The stereochemistry of the hydroperoxide is almost exclusively R (93-94%) Nostoc sp.
A162I the recombinant carboxy-terminal domain of the mutant enzyme A162I produces almost exlusively (13S)-hydroperoxy octadecadienoic acid (90%) Nostoc sp.
A162V the recombinant carboxy-terminal domain of the mutant enzyme A162V converts linoleate primarily to (13S)-hydroperoxy octadecadienoic acid (64%) and to a lesser extent to (9R,10EZ,12Z)-9-hydroperoxy-10,12-octadecadienoate (36%) Nostoc sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
linoleate pH 7.5, 22°C, wild-type enzyme Nostoc sp.
0.005
-
linoleate pH 7.5, 22°C, mutant enzyme A162V Nostoc sp.
0.0056
-
linoleate pH 7.5, 22°C, mutant enzyme A162I Nostoc sp.
0.0089
-
linoleate pH 7.5, 22°C, mutant enzyme A162G Nostoc sp.
0.026
-
alpha-linolenate pH 7.5, 22°C, wild-type enzyme Nostoc sp.

Organism

Organism UniProt Comment Textmining
Nostoc sp.
-
-
-
Nostoc sp. PCC 7120
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant carboxy-terminal domain is purified after expression in Escherichia coli Nostoc sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenate + O2 i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate Nostoc sp. (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
?
alpha-linolenate + O2 i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate Nostoc sp. PCC 7120 (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
?
linoleate + O2 i.e. (9Z,12Z)-octadeca-9,12-dienoate Nostoc sp. (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate configuration of the 9-hydroperoxide is primarily R (93%). (13S)-Hydroperoxy octadecadienoic acid is detected in small amounts as by-product ?
linoleate + O2 i.e. (9Z,12Z)-octadeca-9,12-dienoate Nostoc sp. PCC 7120 (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate configuration of the 9-hydroperoxide is primarily R (93%). (13S)-Hydroperoxy octadecadienoic acid is detected in small amounts as by-product ?

Synonyms

Synonyms Comment Organism
9R-lipoxygenase
-
Nostoc sp.
NspLOX
-
Nostoc sp.