Cloned (Comment) | Organism |
---|---|
recombinant carboxy-terminal domain is purified after expression in Escherichia coli | Nostoc sp. |
Protein Variants | Comment | Organism |
---|---|---|
A162G | the recombinant carboxy-terminal domain of the wild-type enzyme and of mutant enzyme A162G produce primarily (9R,10EZ,12Z)-9-hydroperoxy-10,12-octadecadienoate from linoleate. The stereochemistry of the hydroperoxide is almost exclusively R (93-94%) | Nostoc sp. |
A162I | the recombinant carboxy-terminal domain of the mutant enzyme A162I produces almost exlusively (13S)-hydroperoxy octadecadienoic acid (90%) | Nostoc sp. |
A162V | the recombinant carboxy-terminal domain of the mutant enzyme A162V converts linoleate primarily to (13S)-hydroperoxy octadecadienoic acid (64%) and to a lesser extent to (9R,10EZ,12Z)-9-hydroperoxy-10,12-octadecadienoate (36%) | Nostoc sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
linoleate | pH 7.5, 22°C, wild-type enzyme | Nostoc sp. | |
0.005 | - |
linoleate | pH 7.5, 22°C, mutant enzyme A162V | Nostoc sp. | |
0.0056 | - |
linoleate | pH 7.5, 22°C, mutant enzyme A162I | Nostoc sp. | |
0.0089 | - |
linoleate | pH 7.5, 22°C, mutant enzyme A162G | Nostoc sp. | |
0.026 | - |
alpha-linolenate | pH 7.5, 22°C, wild-type enzyme | Nostoc sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. | - |
- |
- |
Nostoc sp. PCC 7120 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant carboxy-terminal domain is purified after expression in Escherichia coli | Nostoc sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | Nostoc sp. | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | Nostoc sp. PCC 7120 | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
linoleate + O2 | i.e. (9Z,12Z)-octadeca-9,12-dienoate | Nostoc sp. | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | configuration of the 9-hydroperoxide is primarily R (93%). (13S)-Hydroperoxy octadecadienoic acid is detected in small amounts as by-product | ? | |
linoleate + O2 | i.e. (9Z,12Z)-octadeca-9,12-dienoate | Nostoc sp. PCC 7120 | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | configuration of the 9-hydroperoxide is primarily R (93%). (13S)-Hydroperoxy octadecadienoic acid is detected in small amounts as by-product | ? |
Synonyms | Comment | Organism |
---|---|---|
9R-lipoxygenase | - |
Nostoc sp. |
NspLOX | - |
Nostoc sp. |