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Literature summary for 1.13.11.60 extracted from

  • Su, C.; Sahlin, M.; Oliw, E.H.
    A protein radical and ferryl intermediates are generated by linoleate diol synthase, a ferric hemeprotein with dioxygenase and hydroperoxide isomerase activities (1998), J. Biol. Chem., 273, 20744-20751.
    View publication on PubMed
Search for more literature for 1.13.11.60

Inhibitors

Inhibitors Comment Organism Structure
additional information catalysis declines due to suicide inactivation; catalysis declines due to suicide inactivation. Following column chromatography, e.g. ion exchange or zinc-affinity chromatography, hydroperoxide isomerase activity is often decreased and sometimes even undetectable by TLC, whereas 8-dioxygenase activity is always present. However, storage of the enzyme for a few days on ice often restores the hydroperoxide isomerase and appeares to increase the 7,8-linoleate diol synthase activity as well Gaeumannomyces graminis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Gaeumannomyces graminis
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 (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
linoleate + O2 Gaeumannomyces graminis var. graminis
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 (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
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 ?

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis
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-
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Gaeumannomyces graminis Q9UUS2
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Gaeumannomyces graminis var. graminis
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-
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Gaeumannomyces graminis var. graminis Q9UUS2
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-

Purification (Commentary)

Purification (Comment) Organism
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 Gaeumannomyces graminis

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
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 Gaeumannomyces graminis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
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 Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
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 ?
linoleate + O2 bifunctional enzyme with linoleic acid (8R)-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
linoleate + O2 bifunctional enzyme with linoleic acid 8R-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
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 ?
linoleate + O2
-
 Gaeumannomyces graminis var. graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
linoleate + O2 bifunctional enzyme with linoleic acid (8R)-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group Gaeumannomyces graminis var. graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
linoleate + O2 bifunctional enzyme with linoleic acid 8R-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group Gaeumannomyces graminis var. graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?

Cofactor

Cofactor Comment Organism Structure
heme ferric hemeprotein Gaeumannomyces graminis