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Literature summary for 1.13.11.6 extracted from
- Purification and inactivation of 3-hydroxyanthranilic acid 3,4-dioxygenase from beef liver (2003), Int. J. Biochem. Cell Biol., 35, 1085-1097.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-chloro-3-hydroxyanthranilic acid | 5-20 mM, loss of enzymatic activity as a function of the inhibitor concentration | Bos taurus |  |
| anthranilic acid | competitive inhibition | Bos taurus | |
| quinolinic acid | competitive inhibition | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | 19 | 3-Hydroxyanthranilate | - |
Bos taurus | |
| 36 | 57 | 3-hydroxy-4-methylanthranilic acid | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32520 | - |
PAGE in nondenaturing conditions, pI 4.98 enzyme | Bos taurus |
| 32570 | - |
PAGE in nondenaturing conditions, pI 5.6 enzyme | Bos taurus |
| 33000 | - |
SDS-PAGE, for the two active enzyme solutions obtained from hydroxyapatite column | Bos taurus |
| 33000 | 34000 | gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxyanthranilate + O2 | Bos taurus | - |
2-amino-3-carboxymuconate semialdehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| succesive size exclusion and affinity columns | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 115.7 | - |
last purification step | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxy-4-methylanthranilic acid + O2 | - |
Bos taurus | ? | - |
? | |
| 3-hydroxyanthranilate + O2 | - |
Bos taurus | 2-amino-3-carboxymuconate semialdehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000-34000, gel filtration | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
4 | stable for a month, in Tris-maleate buffer, pH 6.5 | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 7.4 | - |
Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
quinolinic acid | for the pI 5.6 enzyme | Bos taurus | |
| 2 | - |
anthranilic acid | for the pI 4.98 enzyme | Bos taurus | |
| 4.9 | - |
anthranilic acid | for the pI 4.98 enzyme | Bos taurus | |
| 6.5 | - |
anthranilic acid | for the pI 5.6 enzyme | Bos taurus | |
| 12 | - |
6-chloro-3-hydroxyanthranilic acid | - |
Bos taurus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bos taurus | chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4 | - |
4.98 |
| Bos taurus | chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4 | - |
5.6 |
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