Cloned (Comment) | Organism |
---|---|
hexahistidine tag, expressed in Escherichia coli BL21 | Xanthomonas campestris |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion, 18°C, PEG 1000 | Xanthomonas campestris |
Protein Variants | Comment | Organism |
---|---|---|
H55A | active site, tryptophan binding | Xanthomonas campestris |
H55S | active site, tryptophan binding | Xanthomonas campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | inhibition of mutant forms H55A and H55S at high concentrations | Xanthomonas campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
5-fluoro-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
0.114 | - |
L-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
0.119 | - |
O2 | - |
Xanthomonas campestris | |
0.133 | - |
L-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.183 | - |
5-fluoro-DL-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.186 | - |
6-fluoro-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
0.194 | - |
5-fluoro-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.195 | - |
6-fluoro-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.197 | - |
L-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.357 | - |
5-methyl-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
0.386 | - |
6-methyl-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.395 | - |
5-methyl-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.546 | - |
6-fluoro-DL-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.975 | - |
6-methyl-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
1.302 | - |
5-methyl-DL-tryptophan | H55S mutant enzyme | Xanthomonas campestris | |
1.98 | - |
6-methyl-DL-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
45.7 | - |
O2 | H55A mutant enzyme | Xanthomonas campestris | |
99 | - |
O2 | H55S mutant enzyme | Xanthomonas campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | Q8PDA8 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-affinity and gel filtration chromatography | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluoro-DL-tryptophan + O2 | - |
Xanthomonas campestris | ? | - |
? | |
5-methyl-DL-tryptophan + O2 | - |
Xanthomonas campestris | ? | - |
? | |
6-fluoro-DL-tryptophan + O2 | - |
Xanthomonas campestris | ? | - |
? | |
6-methyl-DL-tryptophan + O2 | - |
Xanthomonas campestris | ? | - |
? | |
L-tryptophan + O2 | - |
Xanthomonas campestris | N-formylkynurenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Xanthomonas campestris |
Synonyms | Comment | Organism |
---|---|---|
tryptophan 2,3-dioxygenase | - |
Xanthomonas campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
5-methyl-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.68 | - |
5-fluoro-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
0.8 | - |
5-fluoro-DL-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
1.45 | - |
5-methyl-DL-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
1.65 | - |
6-methyl-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
2.4 | - |
5-fluoro-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
2.6 | - |
L-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
2.86 | - |
L-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
3.59 | - |
5-methyl-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
3.78 | - |
6-fluoro-DL-tryptophan | H55A mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
3.8 | - |
6-fluoro-DL-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
3.86 | - |
L-tryptophan | H55A mutant enzyme | Xanthomonas campestris | |
4.66 | - |
6-methyl-DL-tryptophan | H55S mutant enzyme, O2 not present at a saturating level | Xanthomonas campestris | |
7.88 | - |
L-tryptophan | H55S mutant enzyme | Xanthomonas campestris | |
19.5 | - |
L-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
35.4 | - |
L-tryptophan | - |
Xanthomonas campestris | |
37.3 | - |
6-fluoro-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris | |
41 | - |
6-methyl-DL-tryptophan | O2 not present at a saturating level | Xanthomonas campestris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Xanthomonas campestris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
O2 | H55S mutant enzyme | Xanthomonas campestris | |
1.38 | - |
O2 | H55A mutant enzyme | Xanthomonas campestris |