Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.52 extracted from

  • Thackray, S.J.; Bruckmann, C.; Anderson, J.L.; Campbell, L.P.; Xiao, R.; Zhao, L.; Mowat, C.G.; Forouhar, F.; Tong, L.; Chapman, S.K.
    Histidine 55 of tryptophan 2,3-dioxygenase is not an active site base but regulates catalysis by controlling substrate binding (2008), Biochemistry, 47, 10677-10684.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
hexahistidine tag, expressed in Escherichia coli BL21 Xanthomonas campestris

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion, 18°C, PEG 1000 Xanthomonas campestris

Protein Variants

Protein Variants Comment Organism
H55A active site, tryptophan binding Xanthomonas campestris
H55S active site, tryptophan binding Xanthomonas campestris

Inhibitors

Inhibitors Comment Organism Structure
O2 inhibition of mutant forms H55A and H55S at high concentrations Xanthomonas campestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
5-fluoro-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris
0.114
-
L-tryptophan O2 not present at a saturating level Xanthomonas campestris
0.119
-
O2
-
Xanthomonas campestris
0.133
-
L-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.183
-
5-fluoro-DL-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.186
-
6-fluoro-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris
0.194
-
5-fluoro-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.195
-
6-fluoro-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.197
-
L-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.357
-
5-methyl-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris
0.386
-
6-methyl-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.395
-
5-methyl-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.546
-
6-fluoro-DL-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.975
-
6-methyl-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris
1.302
-
5-methyl-DL-tryptophan H55S mutant enzyme Xanthomonas campestris
1.98
-
6-methyl-DL-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
45.7
-
O2 H55A mutant enzyme Xanthomonas campestris
99
-
O2 H55S mutant enzyme Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris Q8PDA8
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity and gel filtration chromatography Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluoro-DL-tryptophan + O2
-
Xanthomonas campestris ?
-
?
5-methyl-DL-tryptophan + O2
-
Xanthomonas campestris ?
-
?
6-fluoro-DL-tryptophan + O2
-
Xanthomonas campestris ?
-
?
6-methyl-DL-tryptophan + O2
-
Xanthomonas campestris ?
-
?
L-tryptophan + O2
-
Xanthomonas campestris N-formylkynurenine
-
?

Subunits

Subunits Comment Organism
homotetramer
-
Xanthomonas campestris

Synonyms

Synonyms Comment Organism
tryptophan 2,3-dioxygenase
-
Xanthomonas campestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
5-methyl-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.68
-
5-fluoro-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
0.8
-
5-fluoro-DL-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
1.45
-
5-methyl-DL-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
1.65
-
6-methyl-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
2.4
-
5-fluoro-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris
2.6
-
L-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
2.86
-
L-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
3.59
-
5-methyl-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris
3.78
-
6-fluoro-DL-tryptophan H55A mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
3.8
-
6-fluoro-DL-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
3.86
-
L-tryptophan H55A mutant enzyme Xanthomonas campestris
4.66
-
6-methyl-DL-tryptophan H55S mutant enzyme, O2 not present at a saturating level Xanthomonas campestris
7.88
-
L-tryptophan H55S mutant enzyme Xanthomonas campestris
19.5
-
L-tryptophan O2 not present at a saturating level Xanthomonas campestris
35.4
-
L-tryptophan
-
Xanthomonas campestris
37.3
-
6-fluoro-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris
41
-
6-methyl-DL-tryptophan O2 not present at a saturating level Xanthomonas campestris

Cofactor

Cofactor Comment Organism Structure
heme
-
Xanthomonas campestris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.48
-
O2 H55S mutant enzyme Xanthomonas campestris
1.38
-
O2 H55A mutant enzyme Xanthomonas campestris