Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-indoleethanol | 1 mM, enhances activity by 87% | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-indoleethanol | lowers Km value for D-tryptophan by 25% at pH 7, enhances Vmax by 40-60% | Oryctolagus cuniculus | |
azide | - |
Oryctolagus cuniculus | |
CO | - |
Oryctolagus cuniculus | |
cyanide | heme ligand, competitive for the ferric enzyme | Oryctolagus cuniculus | |
indole | lowers Km value for D-tryptophan by 60% at pH 7, lowers Vmax by 12-24%, at 1 mM lowers activity by 13% | Oryctolagus cuniculus | |
additional information | 3-indoleethanol and indole affect the L-tryptophan affinity of the ferrous enzyme, indole enhances affinity of enzyme for cyanide and azide and norharman, L-tryptophan lowers norharman affinity for the ferrous dioxygenase | Oryctolagus cuniculus | |
NO | - |
Oryctolagus cuniculus | |
norharman | can bind to the ferric and ferrous enzyme to from a quarternary complex | Oryctolagus cuniculus | |
O2 | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
L-tryptophan | pH 6.0 | Oryctolagus cuniculus | |
0.66 | - |
D-tryptophan | pH 7.0, 1 mM indole | Oryctolagus cuniculus | |
1.3 | - |
D-tryptophan | pH 7.0, 1 mM 3-indoleethanol | Oryctolagus cuniculus | |
1.7 | - |
D-tryptophan | pH 7.0 | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | Oryctolagus cuniculus | - |
N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | Oryctolagus cuniculus | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
highly autoxidizable, requires superoxide anion or a reductant such as ascorbic acid and methylene blue as cofactors | Oryctolagus cuniculus |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
small intestine | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | - |
Oryctolagus cuniculus | N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | - |
Oryctolagus cuniculus | N-formyl-L-kynurenine | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | ascorbic acid in combination with methylene blue or toluidine blue | Oryctolagus cuniculus | |
additional information | superoxide anion in combination with methylene blue or toluidine blue | Oryctolagus cuniculus |