Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.52 extracted from

  • Ishimura, Y.
    L-Tryptophan 2,3-dioxygenase (tryptophan pyrrolase) (Pseudomonas fluorescens) (1970), Methods Enzymol., 17A, 429-434.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
alpha-methyl-DL-tryptophan can activate the enzyme when lower concentrations of L-Trp are used Pseudomonas fluorescens
ascorbic acid the ferric enzyme is incapable of catalyzing the reaction but is reduced to an active ferrous form by the addition of ascorbic acid and L-Trp in presence of O2 Pseudomonas fluorescens
Cys can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
DTT can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
ethylhydroperoxide can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
GSH can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
H2O2 can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens
methylhydroperoxide can reduce the inactive ferric form of the enzyme to the active ferrous form Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
ferricyanide
-
Pseudomonas fluorescens
hydroxylamine
-
Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
O2
-
Pseudomonas fluorescens
0.3
-
L-Trp
-
Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
copper contains copper in significant quantity Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3
-
-
Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
0°C, under anaerobic conditions in presence of tryptophan, stable for several days Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Trp + O2 absolutely specific for L-Trp Pseudomonas fluorescens L-formylkynurenine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Pseudomonas fluorescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
most stable at Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
heme contains protohematin IX as a prosthetic group Pseudomonas fluorescens