Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-methyl-DL-tryptophan | can activate the enzyme when lower concentrations of L-Trp are used | Pseudomonas fluorescens | |
ascorbic acid | the ferric enzyme is incapable of catalyzing the reaction but is reduced to an active ferrous form by the addition of ascorbic acid and L-Trp in presence of O2 | Pseudomonas fluorescens | |
Cys | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
DTT | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
ethylhydroperoxide | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
GSH | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
H2O2 | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens | |
methylhydroperoxide | can reduce the inactive ferric form of the enzyme to the active ferrous form | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ferricyanide | - |
Pseudomonas fluorescens | |
hydroxylamine | - |
Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
O2 | - |
Pseudomonas fluorescens | |
0.3 | - |
L-Trp | - |
Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | contains copper in significant quantity | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
0°C, under anaerobic conditions in presence of tryptophan, stable for several days | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Trp + O2 | absolutely specific for L-Trp | Pseudomonas fluorescens | L-formylkynurenine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
most stable at | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | contains protohematin IX as a prosthetic group | Pseudomonas fluorescens |