Literature summary for 1.13.11.52 extracted from

Dang, Y.; Dale, W.E.; Brown, O.R.
Comparative effects of oxygen on indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase of the kynurenine pathway (2000), Free Radic. Biol. Med., 28, 615-624.

Search for more literature for 1.13.11.52

Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbic acid	tryptophan 2,3-dioxygenase: maximal activity at 10 mM ascorbate	Rattus norvegicus
hyperbaric oxygen	-	Rattus norvegicus
hyperbaric oxygen	-	Meriones unguiculatus
O2-	-	Rattus norvegicus
O2-	-	Meriones unguiculatus

Application

Application	Comment	Organism
medicine	rate-limiting enzyme in kynurenine pathway	Rattus norvegicus
medicine	rate-limiting enzyme in kynurenine pathway	Meriones unguiculatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.8	-	O2	indoleamine 2,3-dioxygenase	Meriones unguiculatus
2.8	-	oxygen	pH 6.5, 37°C	Rattus norvegicus
2.8	-	oxygen	pH 6.5, 37°C	Meriones unguiculatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-tryptophan + O2	Rattus norvegicus	-	N-formyl-D-kynurenine	-	?
D-tryptophan + O2	Meriones unguiculatus	-	N-formyl-D-kynurenine	-	?
L-Trp + O2	Rattus norvegicus	indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid	L-formylkynurenine	-	?
L-Trp + O2	Meriones unguiculatus	indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid	L-formylkynurenine	-	?
L-tryptophan + O2	Rattus norvegicus	no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen	N-formyl-L-kynurenine	-	?
L-tryptophan + O2	Meriones unguiculatus	no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen	N-formyl-L-kynurenine	-	?
L-tryptophan + O2-	Rattus norvegicus	-	N-formyl-L-kynurenine	-	?
L-tryptophan + O2-	Meriones unguiculatus	-	N-formyl-L-kynurenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Meriones unguiculatus	-	-	-
Meriones unguiculatus	-	indoleamine 2,3-dioxygenase	-
Rattus norvegicus	-	Sprague Dawley	-
Rattus norvegicus	-	indoleamine 2,3-dioxygenase from small intestine	-
Rattus norvegicus	-	tryptophan 2,3-dioxygenase from liver	-

Purification (Commentary)

Purification (Comment)	Organism
tissues thawed at room temperature, homogenized in 0.14 M potassium chloride plus 20 mM potassium phosphate, pH 7.0	Rattus norvegicus
tissues thawed at room temperature, homogenized in 0.14 M potassium chloride plus 20 mM potassium phosphate, pH 7.0	Meriones unguiculatus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-
brain	-	Meriones unguiculatus	-
brain	indoleamine 2,3-dioxygenase	Meriones unguiculatus	-
liver	tryptophan 2,3-dioxygenase	Rattus norvegicus	-
lung	-	Meriones unguiculatus	-
lung	indoleamine 2,3-dioxygenase	Meriones unguiculatus	-
small intestine	-	Rattus norvegicus	-
small intestine	indoleamine 2,3-dioxygenase	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.011	-	brain, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Meriones unguiculatus
0.014	-	brain, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Meriones unguiculatus
0.07	-	brain, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Meriones unguiculatus
0.145	-	small intestine, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Rattus norvegicus
0.198	-	lung, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Meriones unguiculatus
0.24	-	small intestine, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Rattus norvegicus
0.38	-	small intestine, in air, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Rattus norvegicus
0.873	-	lung, in 5.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Meriones unguiculatus
0.905	-	lung, in 1.2 atm oxygen, 50 mM potassium phosphate buffer, pH 6.5, 0.025 mM methylene blue, 20 mM ascorbate, 0.05 mM catalase, 0.4 mM L-tryptophan, 37°C	Meriones unguiculatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-tryptophan + O2	-	Rattus norvegicus	N-formyl-D-kynurenine	-	?
D-tryptophan + O2	-	Meriones unguiculatus	N-formyl-D-kynurenine	-	?
L-Trp + O2	tryptophan 2,3-dioxygenase activity is appreciable even at 0.03 mM oxygen and rises steeply to a maximum at 0.04 mM	Rattus norvegicus	L-formylkynurenine	-	?
L-Trp + O2	indoleamine 2,3-dioxygenase has almost no detectable activity at or below 0.1 mM oxygen and maximum activity at about 1.15 mM	Meriones unguiculatus	L-formylkynurenine	-	?
L-Trp + O2	indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid	Rattus norvegicus	L-formylkynurenine	-	?
L-Trp + O2	indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase catalyze the rate-limiting step in the kynurenine pathway from Trp to quinolinic acid	Meriones unguiculatus	L-formylkynurenine	-	?
L-tryptophan + O2	no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen	Rattus norvegicus	N-formyl-L-kynurenine	-	?
L-tryptophan + O2	no activity with oxygen concentrations below 0.1 mM, maximum activity at 1.15 mM oxygen	Meriones unguiculatus	N-formyl-L-kynurenine	-	?
L-tryptophan + O2-	-	Rattus norvegicus	N-formyl-L-kynurenine	-	?
L-tryptophan + O2-	-	Meriones unguiculatus	N-formyl-L-kynurenine	-	?

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Release 2023.1 (January 2023)