Literature summary for 1.13.11.52 extracted from

Littlejohn, T.K.; Takikawa, O.; Skylas, D.; Jamie, J.F.; Walker, M.J.; Truscott, R.J.W.
Expression and purification of recombinant human indoleamine 2,3-dioxygenase (2000), Protein Expr. Purif., 19, 22-29.

Search for more literature for 1.13.11.52

Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbic acid	native and recombinant enzyme require methylene blue and ascorbic acid for activity	Homo sapiens
methylene blue	native and recombinant enzyme require methylene blue and ascorbic acid for activity	Homo sapiens

Application

Application	Comment	Organism
medicine	first and rate-limiting enzyme in tryptophan metabolism, plays a role in pathogenesis of many diseases	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli E538, expressed with His-Tag	Homo sapiens
expression in Escherichia coli EC538 as a fusion protein to a hexahistidyl tag	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	L-tryptophan	-	Homo sapiens
0.02	-	L-Trp	recombinant enzyme	Homo sapiens
0.44	-	5-hydroxy-L-tryptophan	-	Homo sapiens
0.44	-	5-hydroxy-L-tryptophan	recombinant enzyme	Homo sapiens
5	-	D-tryptophan	-	Homo sapiens
5	-	D-Trp	recombinant enzyme	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45440	-	ESI mass spectrometry	Homo sapiens
46980	-	ESI mass spectrometry	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
phosphocellulose and Ni-NTA-agarose affinity chromatography	Homo sapiens
recombinant enzyme, fusion protein to a hexahistidyl tag	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.48	-	kynurenine, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.2 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min	Homo sapiens
2.58	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-hydroxy-L-tryptophan + O2	-	Homo sapiens	N-formyl-5-hydroxy-L-kynurenine	-	?
D-Trp + O2	-	Homo sapiens	N-formyl-D-kynurenine	-	?
D-tryptophan + O2	-	Homo sapiens	N-formyl-D-kynurenine	-	?
L-5-hydroxytryptophan + O2	-	Homo sapiens	?	-	?
L-Trp + O2	-	Homo sapiens	L-formylkynurenine	-	?
L-tryptophan + O2	-	Homo sapiens	N-formyl-L-kynurenine	-	?

Synonyms

Synonyms	Comment	Organism
IDO	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.065	-	5-hydroxy-L-tryptophan	-	Homo sapiens
1.55	-	L-Trp	-	Homo sapiens
2.07	-	D-Trp	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	isoelectric focusing	-	7.09

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Release 2023.1 (January 2023)