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Literature summary for 1.13.11.52 extracted from

  • Matsumura, M.; Osada, K.; Aiba, S.
    L-Tryptophan 2,3-dioxygenase of a moderate thermophile, Bacillus brevis. Purification, properties and a substrate-mediated stabilization of the quaternary structure (1984), Biochim. Biophys. Acta, 786, 9-17.
    View publication on PubMed

General Stability

General Stability Organism
guanidine hydrochloride, 30°C, 30 min, about 15% loss of activity at 0.5 M, about 30% loss of activity at 1 mM, about 60% loss of activity at 2 M, complete loss of activity at 3 mM Brevibacillus brevis
urea, 30°C, 30 min, stable at 2.5 M, about 30% loss of activity at 5.0 M, about 60% loss of activity at 7.5 M Brevibacillus brevis

Inhibitors

Inhibitors Comment Organism Structure
CN- 0.4 mM, 86% inhibition Brevibacillus brevis
CO 20% with 80% air, 78% inhibition Brevibacillus brevis
D-Trp no inhibition Brevibacillus brevis
DL-5-hydroxytryptophan 1 mM, 55% inhibition Brevibacillus brevis
indole 1 mM, 44% inhibition Brevibacillus brevis
Indolepropionic acid 1 mM, 31% inhibition Brevibacillus brevis
K3Fe(CN)6 1 mM, complete inhibition Brevibacillus brevis
N3- 2 mM, 22% inhibition Brevibacillus brevis
NH2OH 2 mM, 54% inhibition Brevibacillus brevis
tryptamine 1 mM, 23% inhibition Brevibacillus brevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
O2
-
Brevibacillus brevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
4 * 27000, SDS-PAGE Brevibacillus brevis

Organism

Organism UniProt Comment Textmining
Brevibacillus brevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brevibacillus brevis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.85
-
-
Brevibacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-5-fluoro-methyltryptophan + O2 35% of the activity with L-Trp Brevibacillus brevis ?
-
?
DL-5-methyltryptophan + O2 90% of the activity with L-Trp Brevibacillus brevis ?
-
?
DL-6-fluorotryptophan + O2 90% of the activity with L-Trp Brevibacillus brevis ?
-
?
DL-6-methyltryptophan 98% of the activity with L-Trp Brevibacillus brevis ?
-
?
L-Trp + O2
-
Brevibacillus brevis L-formylkynurenine
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE Brevibacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Brevibacillus brevis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
L-Trp stabilizes against heat inactivation Brevibacillus brevis
70
-
30 min, stable in presence of 5 mM L-Trp Brevibacillus brevis
75
-
30 min, about 5% loss of activity in presence of 5 mM L-Trp Brevibacillus brevis
80
-
30 min, about 35% loss of activity in presence of 5 mM L-Trp Brevibacillus brevis
85
-
30 min, complete loss of activity in presence of 5 mM L-Trp Brevibacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Brevibacillus brevis

Cofactor

Cofactor Comment Organism Structure
heme contains 1 mol or more of heme per mol of enzyme Brevibacillus brevis