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Literature summary for 1.13.11.51 extracted from

  • Scherzinger, D.; Al-Babili, S.
    In vitro characterization of a carotenoid cleavage dioxygenase from Nostoc sp. PCC 7120 reveals a novel cleavage pattern, cytosolic localization and induction by highlight (2008), Mol. Microbiol., 69, 231-244.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information induction of enzyme activity with highlight treatment Nostoc sp. PCC 7120 = FACHB-418

Cloned(Commentary)

Cloned (Comment) Organism
expression as a glutathion-S-transferase fusion protein in Escherichia coli BL21 Nostoc sp. PCC 7120 = FACHB-418

Inhibitors

Inhibitors Comment Organism Structure
additional information decreased enzyme activtiy at lower temperature Nostoc sp. PCC 7120 = FACHB-418

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Nostoc sp. PCC 7120 = FACHB-418 5829
-
soluble
-
Nostoc sp. PCC 7120 = FACHB-418
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
value about, Western blot Nostoc sp. PCC 7120 = FACHB-418

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418 Q8YXV3 Anabaena sp. PCC 7120
-

Purification (Commentary)

Purification (Comment) Organism
-
Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-OH-beta-apo-10'-carotenal + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 apo-10',10-apocarotene-dial + ?
-
?
3-OH-beta-apo-8'-carotenal + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 apo-8',10-apocarotene-dial
-
?
4-hydroxymyxol 2'-fucoside + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
4-ketomyxol 2'-fucoside + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
astaxanthin + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
beta-apo-10'-carotenal + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
beta-apo-8'-carotenal + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
canthaxanthin + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
echinenone + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
gamma-carotene + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
myxol + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
myxol 2'-fucoside + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?
zeaxanthin + O2 assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone Nostoc sp. PCC 7120 = FACHB-418 ?
-
?

Synonyms

Synonyms Comment Organism
carotenoid cleavage dioxygenase
-
Nostoc sp. PCC 7120 = FACHB-418
NosCCD
-
Nostoc sp. PCC 7120 = FACHB-418
Nostoc carotenoid cleavage dioxygenase
-
Nostoc sp. PCC 7120 = FACHB-418
NSC1
-
Nostoc sp. PCC 7120 = FACHB-418

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
at 20°C decreased enzyme acitivity Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Nostoc sp. PCC 7120 = FACHB-418