Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a homology model by apocarotenoid-15,15'-oxygenase, EC 1.14.99.n1, is used | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1R,3S,6R)-(3'Z)-1,5,5-trimethyl-6-(3'-methyl-5'-(phenylamino)-pent-3'-en-1'-ynyl)-7-oxabicyclo[4.1.0]heptan-3-ol | 1 mM, complete inhibition of enzyme activity | Arabidopsis thaliana | |
(1R,3S,6R)-(3'Z)-1,5,5-trimethyl-6-(3'-methyl-5'-(phenylthio)-pent-3'-en-1'-ynyl)-7-oxa-bicyclo[4.1.0]heptan-3-ol | 1 mM, complete inhibition of enzyme activity | Arabidopsis thaliana | |
(1R,4R,6R)-(3'Z)-1-(5'-(ethylthio)-3'-methylpent-3'-en-1'-ynyl)-2,2,6-trimethylcyclohexane-1,4-diol | 1 mM, about 25% inhibition of enzyme activity | Arabidopsis thaliana | |
(1S,4R,6R)-(3'Z)-1-(5'-(ethylthio)-3'-methylpent-3'-en-1'-ynyl)-2,2,6-trimethylcyclohexane-1,4-diol | 1 mM, about 65% inhibition of enzyme activity | Arabidopsis thaliana | |
(2Z,4E)-5-(1'-hydroxy-2',2',6'-trimethyl-4'-oxocyclohexyl)-3-methylpenta-2,4-dienyl 2-(thiophen-2''-yl)acetate | 1 mM, about 70% inhibition of enzyme activity | Arabidopsis thaliana | |
(4R,5S)-(3'Z)-4-(5'-(ethylthio)-3'-methylpent-3'-en-1'-ynyl)-4-hydroxy-3,3,5-trimethylcyclohexanone | 1 mM, complete inhibition of enzyme activity | Arabidopsis thaliana | |
(4S,5R)-(3'Z)-4-(5'-(ethylthio)-3'-methylpent-3'-en-1'-ynyl)-4-hydroxy-3,3,5-trimethylcyclohexanone | 1 mM, about 55% inhibition of enzyme activity | Arabidopsis thaliana | |
(4S,5R/4R,5S)-(3'Z)-4-(5'-(ethylthio)-3'-methylpent-3'-en-1'-ynyl)-4-hydroxy-3,3,5-trimethylcyclohexanone | 1 mM, 75% inhibition of enzyme activity | Arabidopsis thaliana | |
abamine | 1 mM, about 70% inhibition of enzyme activity | Arabidopsis thaliana | |
abamine SG | 1 mM, about 80% inhibition of enzyme activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
9-cis-neoxanthin | - |
Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione sepharose 4 fast flow resin | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-cis-neoxanthin + O2 | assay at pH 6.7, 22°C, 20 min, reaction stopped by adding 50 microl of 25% Triton X-100 | Arabidopsis thaliana | xanthoxin + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
9-cis-epoxycarotenoid dioxygenase | key enzyme of abscisic acid biosynthesis | Arabidopsis thaliana |
AtNCED3 | - |
Arabidopsis thaliana |
NCED | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
assay at | Arabidopsis thaliana |