General Stability | Organism |
---|---|
ascorbate stabilizes, it probably protects Fe2+ from spontaneous oxidation | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | strong inhibitory effect, but stabilizes | Mus musculus | |
Co2+ | incubation with Fe2+ plus Co2+ in equimolar concentrations inhibits | Mus musculus | |
Cu2+ | incubation with Fe2+ plus Cu2+ in equimolar concentrations inhibits | Mus musculus | |
glutathione | reduced glutathione slightly inhibits | Mus musculus | |
additional information | not inhibited by Ca2+ and Mn2+ | Mus musculus | |
Ni2+ | incubation with Fe2+ plus Ni2+ in equimolar concentrations inhibits | Mus musculus | |
Zn2+ | incubation with Fe2+ plus Zn2+ in equimolar concentrations inhibits | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.188 | - |
homogentisate | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+ is an absolutely obligate cofactor and cannot be replaced by other divalent cations, highest enzyme activity in vitro at 2 mM Fe2+ | Mus musculus | |
additional information | Fe3+ cannot replace Fe2+ | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
3 * 49000, SDS-PAGE | Mus musculus |
149000 | - |
gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | Mus musculus | homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring | 4-maleylacetoacetate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
330fold purification | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | - |
Mus musculus | 4-maleylacetoacetate | - |
ir | |
homogentisate + O2 | homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring | Mus musculus | 4-maleylacetoacetate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 49000, SDS-PAGE | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | almost linear decrease of activity | Mus musculus |
45 | - |
65% loss of activity | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Mus musculus |