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Literature summary for 1.13.11.5 extracted from
- Murine liver homogentisate 1,2-dioxygenase. Purification to homogeneity and novel biochemical properties (1995), Eur. J. Biochem., 228, 425-430.
General Stability
| General Stability | Organism |
|---|---|
| ascorbate stabilizes, it probably protects Fe2+ from spontaneous oxidation | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | strong inhibitory effect, but stabilizes | Mus musculus |  |
| Co2+ | incubation with Fe2+ plus Co2+ in equimolar concentrations inhibits | Mus musculus | |
| Cu2+ | incubation with Fe2+ plus Cu2+ in equimolar concentrations inhibits | Mus musculus | |
| glutathione | reduced glutathione slightly inhibits | Mus musculus | |
| additional information | not inhibited by Ca2+ and Mn2+ | Mus musculus | |
| Ni2+ | incubation with Fe2+ plus Ni2+ in equimolar concentrations inhibits | Mus musculus | |
| Zn2+ | incubation with Fe2+ plus Zn2+ in equimolar concentrations inhibits | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.188 | - |
homogentisate | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Fe2+ is an absolutely obligate cofactor and cannot be replaced by other divalent cations, highest enzyme activity in vitro at 2 mM Fe2+ | Mus musculus | |
| additional information | Fe3+ cannot replace Fe2+ | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
3 * 49000, SDS-PAGE | Mus musculus |
| 149000 | - |
gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homogentisate + O2 | Mus musculus | homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring | 4-maleylacetoacetate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 330fold purification | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homogentisate + O2 | - |
Mus musculus | 4-maleylacetoacetate | - |
ir | |
| homogentisate + O2 | homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring | Mus musculus | 4-maleylacetoacetate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 49000, SDS-PAGE | Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | almost linear decrease of activity | Mus musculus |
| 45 | - |
65% loss of activity | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
- |
Mus musculus |
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