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Literature summary for 1.13.11.49 extracted from

  • Blanc, B.; Rodgers, K.; Lukat-Rodgers, G.; Dubois, J.
    Understanding the roles of strictly conserved tryptophan residues in O2 producing chlorite dismutases (2013), Dalton Trans., 42, 3156-3169.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.49

Protein Variants

Protein Variants Comment Organism
W155F site-directed mutagenesis, mutation of a conserved residue on the proximal side of the heme causes a loss of the characteristic pentameric oligomerization state, secondary structure, and heme binding properties of the wild-type protein. Conversion to an inactive, heme-free form is accelerated by dilution Dechloromonas aromatica
W156F site-directed mutagenesis, mutation of a conserved residue on the proximal side of the heme causes a loss of the characteristic pentameric oligomerization state, secondary structure, and heme binding properties of the wild-type protein. Conversion to an inactive, heme-free form is accelerated by dilution Dechloromonas aromatica
W227F site-directed mutagenesis, mutation of a conserved residue on the proximal side of the heme, but W227F retains many properties of the wild-type protein, the mutant reacts with peracetic acid at pH 6.0-8.0, overview Dechloromonas aromatica

Inhibitors

Inhibitors Comment Organism Structure
additional information suicide inactivation by oxidative heme destruction Dechloromonas aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information transient kinetic studies with wild-type and mutant enzymes, and steady-state kinetics, overview Dechloromonas aromatica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme enzyme Dechloromonas aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28400
-
 2 * 28400, recombinant mutant W155F, SDS-PAGE Dechloromonas aromatica
54000
-
 recombinant mutant W155F, gel filtration Dechloromonas aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorite Dechloromonas aromatica
-
 chloride + O2
-
 ?

Organism

Organism UniProt Comment Textmining
Dechloromonas aromatica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorite
-
 Dechloromonas aromatica chloride + O2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 28400, recombinant mutant W155F, SDS-PAGE Dechloromonas aromatica

Synonyms

Synonyms Comment Organism
chlorite dismutase
-
 Dechloromonas aromatica
CLD
-
 Dechloromonas aromatica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25 assay at Dechloromonas aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 7.9 assay at Dechloromonas aromatica

Cofactor

Cofactor Comment Organism Structure
heme
-
 Dechloromonas aromatica

General Information

General Information Comment Organism
malfunction the W155F mutation of the enzyme appears to disturb both the relatively higher heme affinity and pentameric oligomerization state common to the wild-type enzyme, mutant W156F, and mutant W227F Dechloromonas aromatica