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Literature summary for 1.13.11.49 extracted from

  • Mehboob, F.; Wolterink, A.F.; Vermeulen, A.J.; Jiang, B.; Hagedoorn, P.L.; Stams, A.J.; Kengen, S.W.
    Purification and characterization of a chlorite dismutase from Pseudomonas chloritidismutans (2009), FEMS Microbiol. Lett., 293, 115-121.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
azide at 70 microM 44% inhibition, at 700 microM 89% inhibition Stutzerimonas chloritidismutans
cyanide at 70 microM 83% inhibition, at 700 microM 95% inhibition Stutzerimonas chloritidismutans
EDTA at 70 microM 13% inhibition, at 700 microM 30% inhibition Stutzerimonas chloritidismutans
H2O2 at 70 microM 6% inhibition, at 700 microM 37% inhibition Stutzerimonas chloritidismutans
additional information excess chlorite inactivates and bleaches the enzyme, 80% activity loss with 56 mM chlorite, 90% after second application of 56 mM chlorite; no inhibition by hydroxylamine or 3-amino-1,2,3-triazole Stutzerimonas chloritidismutans

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Stutzerimonas chloritidismutans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 activity increases with increasing salt concentration up to 2 M Stutzerimonas chloritidismutans
additional information kosmotropic salts such as sulfate and phosphate stabilize protein structures by water-structuring effects of the ions, chaotropic salts such as nitrate and chlorate reduce the activity, no effect of NaCl, NH4Cl, and NaHCO3 (up to 1.5 M) on enzyme activity Stutzerimonas chloritidismutans
Na2SO4 activity increases with increasing salt concentration up to 2 M Stutzerimonas chloritidismutans
NaClO3 decreasing activity with increasing salt concentration Stutzerimonas chloritidismutans
NaNO3 decreasing activity with increasing salt concentration Stutzerimonas chloritidismutans
potassium phosphate activity increases with increasing salt concentration up to 1 M salt, decrease beyond 1 M salt Stutzerimonas chloritidismutans
Sodium phosphate activity increases with increasing salt concentration up to 1 M salt, decrease beyond 1 M salt Stutzerimonas chloritidismutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS) Stutzerimonas chloritidismutans
110000
-
SDS-PAGE, native enzyme with Superdex 200 column with 50 mM potassium phosphate buffer, pH 7.0 and 100 mM NaCl Stutzerimonas chloritidismutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorite Stutzerimonas chloritidismutans
-
chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?
chlorite Stutzerimonas chloritidismutans AW-1 / DSM 13592
-
chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?

Organism

Organism UniProt Comment Textmining
Stutzerimonas chloritidismutans
-
-
-
Stutzerimonas chloritidismutans AW-1 / DSM 13592
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cell pellet suspended in 0.05 M Tris-HCl buffer, pH 9.5 and 0.05 EDTA for 30 min at room temperature, centrifuged, supernatant ultracentrifuged, red supernatant containing periplasmic fraction separated from pellet, diluted with 10 mM Tris-HCl buffer, pH 7.2, purification with ÄKTAfplc, diluted supernatant loaded onto hydroxyapatite column with 10 mM Tris-HCl, pH 7.2, enzyme elutes at the start of a linear gradient of 10 mM Tris-Hcl, pH 7.2 to 450 mM potassium phosphate, pH 7.2, storage at -20°C Stutzerimonas chloritidismutans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the formation of O2 is influenced by the concentrations of enzyme and chlorite in 1 M phosphate buffer, pH 6.0, at 25°C, undiluted chlorite dismutase (0.64 mg/l) produces 98 microM O2 with 100 microM ClO2-, 205 microM O2 with 200 microM ClO2-, 300 microM O2 with 300 microM ClO2-, 10 times diluted chlorite dismutase (0.064 mg/l) produces 34 microM O2 with 100 microM ClO2-, 69 microM O2 with 200 microM ClO2-, 96 microM O2 with 300 microM ClO2- Stutzerimonas chloritidismutans
443
-
Vmax, 100 mM sodium phosphate buffer, pH 6.0, 25°C, 0.2 mM chlorite Stutzerimonas chloritidismutans
4650
-
Vmax increase with 1 M phosphate, pH 6.0, 25°C Stutzerimonas chloritidismutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorite
-
Stutzerimonas chloritidismutans chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?
chlorite
-
Stutzerimonas chloritidismutans AW-1 / DSM 13592 chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?

Subunits

Subunits Comment Organism
homotetramer 4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS) Stutzerimonas chloritidismutans

Synonyms

Synonyms Comment Organism
chlorite dismutase
-
Stutzerimonas chloritidismutans
CLD
-
Stutzerimonas chloritidismutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
100 mM sodium phosphate buffer, pH 7.2 Stutzerimonas chloritidismutans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45 100 mM sodium phosphate buffer, pH 7.2 Stutzerimonas chloritidismutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
sodium phosphate buffer, 25°C Stutzerimonas chloritidismutans

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 sodium phosphate buffer, 25°C Stutzerimonas chloritidismutans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2700
-
Chlorite 100 mM sodium phosphate buffer, pH 6.0, 25°C Stutzerimonas chloritidismutans