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Literature summary for 1.13.11.45 extracted from

  • Cristea, M.; Oliw, E.H.
    On the singular, dual, and multiple positional specificity of manganese lipoxygenase and its G316A mutant (2007), J. Lipid Res., 48, 890-903.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and G316A mutant enzymes in Pichia pastoris strain X-33, secretion to the cell medium Gaeumannomyces graminis

Protein Variants

Protein Variants Comment Organism
G316A site-directed mutagenesis, the mutant MnIII-LO G316A oxygenates mainly 16:3n-3 at positions n-7 and n-6, 19:3n-3 at n-10, n-8, and n-6, and 20:3n-3 at n-10 and n-8 Gaeumannomyces graminis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ a Mn-lipoxygenase Gaeumannomyces graminis

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Pichia pastoris cell medium by hydrophobic interaction and ion exchange chromatography Gaeumannomyces graminis

Reaction

Reaction Comment Organism Reaction ID
linoleate + O2 = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate reaction mechanism, tail-first model with rearrangement at C13 at the end of lipoxygenation, influenced by double bond configuration and the chain length of fatty acids, overview Gaeumannomyces graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(10Z,13Z,16Z)-nonadeca-10,13,16-trienoic acid + O2 oxidation at the n-6 carbon and at the bis-allylic position n-8 Gaeumannomyces graminis ?
-
?
(11Z,14Z,17Z)-eicosa-11,14,17-trienoic acid + O2 oxidation at the n-6 carbon and at the bis-allylic position n-8 Gaeumannomyces graminis ?
-
?
(5E,8E,11E,14E)-icosa-5,8,11,14-tetraenoic acid + O2 oxidation at the n-6 carbon and at the bis-allylic position n-8 Gaeumannomyces graminis (5Z,8Z,11S,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8Z,11Z,13E,15R)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid
-
?
(5Z,8Z,11Z,14Z,17Z)-docosa-5,8,11,14,17-pentaenoate + O2 oxidation at the n-6 carbon and at the bis-allylic position n-8 Gaeumannomyces graminis ?
-
?
(7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoate + O2 oxidation at the n-6 carbon and at the bis-allylic position n-8 Gaeumannomyces graminis ?
-
?
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 via bis-allylic 11S-hydroperoxy fatty acid Gaeumannomyces graminis (13R)-hydroperoxyoctadecadienoic acid
-
?
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 oxidation at the bis-allylic n-5 carbon and at positions n-3, n-7, and n-6 Gaeumannomyces graminis ?
-
?
1,2-dilinoleoyl-sn-glycero-3-phosphocholine + O2
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Gaeumannomyces graminis ? main products with 14% and 10%, no production of 11-hydroxyoctadecanoic acid ?
linoleate + O2 via bis-allylic 11S-hydroperoxy fatty acid Gaeumannomyces graminis (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate
-
?
additional information Mn-LO likely binds fatty acids tail-first and oxygenates many C16, C18, C20, and C22 fatty acids to significant amounts of bis-allylic hydroperoxides. Unsaturated C16-C22 fatty acids, other than 18:3n-3, 18:2n-6, or 17:3n-3, are poor substrates, possibly because of ineffective enzyme activation through Mn2+ to Mn3+ by the produced hydroperoxides, substrate specificities of wild-type and mutant enzymes, overview Gaeumannomyces graminis ?
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?

Synonyms

Synonyms Comment Organism
manganese lipoxygenase
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Gaeumannomyces graminis
Mn-LO
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Gaeumannomyces graminis