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Literature summary for 1.13.11.45 extracted from

  • Cristea, M.; Engstrom, K.; Su, C.; Hornsten, L.; Oliw, E.H.
    Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands (2005), Arch. Biochem. Biophys., 434, 201-211.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H274Q no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
H278E no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
H462E no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
H463Q no significant change in activity Gaeumannomyces graminis
additional information V602 deletion mutant, no enzymic activity, loss of more than 95% of manganese content Gaeumannomyces graminis
N466L no significant change in activity Gaeumannomyces graminis
N466Q no significant change in activity Gaeumannomyces graminis
Q467N no significant change in activity Gaeumannomyces graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0071
-
alpha-linolenic acid pH 9.0, 21┬░C Gaeumannomyces graminis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mol per mol of protein Gaeumannomyces graminis

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis
-
expression in Pichia pastoris
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-and O-glycosilation Gaeumannomyces graminis

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Gaeumannomyces graminis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
18
-
pH 9.0, 21┬░C Gaeumannomyces graminis

Storage Stability

Storage Stability Organism
4┬░C, stable for months Gaeumannomyces graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenic acid + O2
-
Gaeumannomyces graminis ?
-
?

Subunits

Subunits Comment Organism
? x * 80-100000, SDS-PAGE Gaeumannomyces graminis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Gaeumannomyces graminis

pH Range

pH Minimum pH Maximum Comment Organism
7
-
93% of maximum activity Gaeumannomyces graminis
8
-
98% of maximum activity Gaeumannomyces graminis