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Literature summary for 1.13.11.45 extracted from

  • Su, C.; Oliw, E.H.
    Manganese lipoxygenase. Purification and characterization (1998), J. Biol. Chem., 273, 13072-13079.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
arachidonic acid inhibits at 0.01 mM Gaeumannomyces graminis
eicosatetraynoic acid 50% of control activity at 0.01 mM Gaeumannomyces graminis
additional information no inhibition by lipoxygenase inhibitors zileuton, esculetin and ICI 230487 Gaeumannomyces graminis
N-(3-phenoxycinnamyl)acetohydroxamic acid 50% of control activity at 0.05 mM Gaeumannomyces graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
alpha-linolenic acid
-
Gaeumannomyces graminis
0.0044
-
linoleic acid
-
Gaeumannomyces graminis
0.031
-
O2
-
Gaeumannomyces graminis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ contains 0.5-1.0 atom per enzyme molecule Gaeumannomyces graminis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
SDS-PAGE after deglycosylation Gaeumannomyces graminis
135000
-
gel filtration with Superdex 200 in 6 M urea Gaeumannomyces graminis
160000
-
gel filtration with Superdex 200 Gaeumannomyces graminis

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis
-
take-all fungus
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Gaeumannomyces graminis

Purification (Commentary)

Purification (Comment) Organism
-
Gaeumannomyces graminis

Storage Stability

Storage Stability Organism
4┬░C, 3 mM NaN3, 0.2 mM GSH, several months, no loss of activity Gaeumannomyces graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenic acid + O2
-
Gaeumannomyces graminis (9Z,11E,15Z)-(13)-hydroperoxyoctadecatrienoic acid
-
?
gamma-linolenic acid + O2
-
Gaeumannomyces graminis (6Z,9Z,11E)-(13)-hydroperoxyoctadecatrienoic acid + (6Z,9Z,12Z)-(11)-hydroperoxyoctadecatrienoic acid
-
?
linoleic acid + O2
-
Gaeumannomyces graminis (9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
60
-
-
Gaeumannomyces graminis

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
12 60 8.8% of maximal activity at 12┬░C and 100% of maximal activity at 60┬░C, activity decreases sharply at 63┬░C Gaeumannomyces graminis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
63
-
incubation for 5 min over 63┬░C decreases activity, no activity at 75┬░C Gaeumannomyces graminis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.3
-
linoleic acid
-
Gaeumannomyces graminis
23.3
-
O2
-
Gaeumannomyces graminis
40
-
alpha-linolenic acid
-
Gaeumannomyces graminis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
broad pH optimum Gaeumannomyces graminis

pH Range

pH Minimum pH Maximum Comment Organism
5 11 at pH 5.0 64% of maximum activity, at pH 11 69% of maximum activity Gaeumannomyces graminis